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タイトル: | Graft reconditioning with nitric oxide gas in rat liver transplantation from cardiac death donors. |
著者: | Kageyama, Shoichi Yagi, Shintaro https://orcid.org/0000-0001-7465-5761 (unconfirmed) Tanaka, Hirokazu Saito, Shunichi Nagai, Kazuyuki Hata, Koichiro https://orcid.org/0000-0002-3609-6396 (unconfirmed) Fujimoto, Yasuhiro Ogura, Yasuhiro Tolba, Rene Shinji, Uemoto |
著者名の別形: | 影山, 詔一 八木, 真太郎 |
キーワード: | Cardiac death donor Organ preservation Ischemia-reperfusion injury Warm ischemia Liver transplantation |
発行日: | 27-Mar-2014 |
出版者: | Lippincott Williams & Wilkins |
誌名: | Transplantation |
巻: | 97 |
号: | 6 |
開始ページ: | 618 |
終了ページ: | 625 |
抄録: | Background: Liver transplant outcomes using grafts donated after cardiac death (DCD) remain poor. Methods: We investigated the effects of ex vivo reconditioning of DCD grafts with venous systemic oxygen persufflation using nitric oxide gas (VSOP-NO) in rat liver transplants. Orthotopic liver transplants were performed in Lewis rats, using DCD grafts prepared using static cold storage alone (group-control) or reconditioning using VSOP-NO during cold storage (group-VSOP-NO). Experiment I: In a 30-min warm ischemia model, graft damage and hepatic expression of inflammatory cytokines, endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), and endothelin-1 (ET-1) were examined, and histologic analysis was performed 2, 6, 24, and 72 hr after transplantation. Experiment II: In a 60-min warm ischemia model, grafts were evaluated 2 hr after transplantation (6 rats/group), and survival was assessed (7 rats/group). Results: Experiment I: Group-VSOP-NO had lower alanine aminotransferase (ALT) (P<0.001), hyaluronic acid (P<0.05), and malondialdehyde (MDA) (P<0.001), hepatic interleukin-6 expression (IL-6) (P<0.05), and hepatic tumor necrosis factor-alpha (TNF-[alpha]) expression (P<0.001). Hepatic eNOS expression (P<0.001) was upregulated, whereas hepatic iNOS (P<0.01) and ET-1 (P<0.001) expressions were downregulated. The damage of hepatocyte and sinusoidal endothelial cells (SECs) were lower in group-VSOP-NO. Experiment II: VSOP-NO decreased ET-1 and 8-hydroxy-2'deoxyguanosine (8-OHdG) expression and improved survival after transplantation by 71.4% (P<0.01). Conclusion: These results suggest that VSOP-NO effectively reconditions warm ischemia-damaged grafts, presumably by decreasing ET-1 upregulation and oxidative damage. |
著作権等: | © 2014 by Lippincott Williams & Wilkins この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/198289 |
DOI(出版社版): | 10.1097/TP.0000000000000025 |
PubMed ID: | 24521773 |
出現コレクション: | 学術雑誌掲載論文等 |
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