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タイトル: | Replications of Two Closely Related Groups of Jumbo Phages Show Different Level of Dependence on Host-encoded RNA Polymerase |
著者: | Matsui, Takeru Yoshikawa, Genki Mihara, Tomoko Chatchawankanphanich, Orawan Kawasaki, Takeru Nakano, Miyako Fujie, Makoto Ogata, Hiroyuki https://orcid.org/0000-0001-6594-377X (unconfirmed) Yamada, Takashi |
著者名の別形: | 吉川, 元貴 三原, 知子 緒方, 博之 |
キーワード: | jumbo phages ΦKZ-like phages Ralstonia solanacearum genomic analysis virion-associated-RNA polymerase |
発行日: | 13-Jun-2017 |
出版者: | Frontiers Media SA |
誌名: | Frontiers in Microbiology |
巻: | 8 |
論文番号: | 1010 |
抄録: | Ralstonia solanacearum phages ΦRP12 and ΦRP31 are jumbo phages isolated in Thailand. Here we show that they exhibit similar virion morphology, genome organization and host range. Genome comparisons as well as phylogenetic and proteomic tree analyses support that they belong to the group of ΦKZ-related phages, with their closest relatives being R. solanacearum phages ΦRSL2 and ΦRSF1. Compared with ΦRSL2 and ΦRSF1, ΦRP12 and ΦRP31 possess larger genomes (ca. 280 kbp, 25% larger). The replication of ΦRP12 and ΦRP31 was not affected by rifampicin treatment (20 μg/ml), suggesting that phage-encoded RNAPs function to start and complete the infection cycle of these phages without the need of host-encoded RNAPs. In contrast, ΦRSL2 and ΦRSF1, encoding the same set of RNAPs, did not produce progeny phages in the presence of rifampicin (5 μg/ml). This observation opens the possibility that some ΦRP12/ΦRP31 factors that are absent in ΦRSL2 and ΦRSF1 are involved in their host-independent transcription. |
著作権等: | © 2017 Matsui, Yoshikawa, Mihara, Chatchawankanphanich, Kawasaki, Nakano, Fujie, Ogata and Yamada. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
URI: | http://hdl.handle.net/2433/226642 |
DOI(出版社版): | 10.3389/fmicb.2017.01010 |
PubMed ID: | 28659872 |
出現コレクション: | 学術雑誌掲載論文等 |
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