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ファイル | 記述 | サイズ | フォーマット | |
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j.celrep.2017.12.057.pdf | 4.23 MB | Adobe PDF | 見る/開く |
タイトル: | Identification of Cardiomyocyte-Fated Progenitors from Human-Induced Pluripotent Stem Cells Marked with CD82 |
著者: | Takeda, Masafumi Kanki, Yasuharu Masumoto, Hidetoshi https://orcid.org/0000-0002-4059-2827 (unconfirmed) Funakoshi, Shunsuke Hatani, Takeshi Fukushima, Hiroyuki Izumi-Taguchi, Akashi Matsui, Yusuke Shimamura, Teppei Yoshida, Yoshinori https://orcid.org/0000-0001-5511-9090 (unconfirmed) Yamashita, Jun K. |
著者名の別形: | 武田, 匡史 升本, 英利 舟越, 俊介 羽渓, 健 吉田, 善紀 山下, 潤 |
キーワード: | iPSCs CD82 cardiomyocytes progenitors exosome Wnt inhibition |
発行日: | 9-Jan-2018 |
出版者: | Elsevier B.V. |
誌名: | Cell Reports |
巻: | 22 |
号: | 2 |
開始ページ: | 546 |
終了ページ: | 556 |
抄録: | Here, we find that human-induced pluripotent stem cell (hiPSC)-derived cardiomyocyte (CM)-fated progenitors (CFPs) that express a tetraspanin family glycoprotein, CD82, almost exclusively differentiate into CMs both in vitro and in vivo. CD82 is transiently expressed in late-stage mesoderm cells during hiPSC differentiation. Purified CD82⁺ cells gave rise to CMs under nonspecific in vitro culture conditions with serum, as well as in vivo after transplantation to the subrenal space or injured hearts in mice, indicating that CD82 successfully marks CFPs. CD82 overexpression in mesoderm cells as well as in undifferentiated hiPSCs increased the secretion of exosomes containing β-catenin and reduced nuclear β-catenin protein, suggesting that CD82 is involved in fated restriction to CMs through Wnt signaling inhibition. This study may contribute to the understanding of CM differentiation mechanisms and to cardiac regeneration strategies. |
著作権等: | © 2017 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
URI: | http://hdl.handle.net/2433/230781 |
DOI(出版社版): | 10.1016/j.celrep.2017.12.057 |
PubMed ID: | 29320747 |
出現コレクション: | 学術雑誌掲載論文等 |
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