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タイトル: | High-throughput screening in colorectal cancer tissue-originated spheroids |
著者: | Kondo, Jumpei Ekawa, Tomoya Endo, Hiroko Yamazaki, Kanami Tanaka, Norio Kukita, Yoji Okuyama, Hiroaki Okami, Jiro Imamura, Fumio Ohue, Masayuki Kato, Kikuya Nomura, Taisei Kohara, Arihiro Mori, Seiichi Dan, Shingo Inoue, Masahiro https://orcid.org/0000-0001-7315-026X (unconfirmed) |
著者名の別形: | 近藤, 純平 井上, 正宏 |
キーワード: | 3D culture cancer heterogeneity high throughput screening organoid |
発行日: | Jan-2019 |
出版者: | Wiley |
誌名: | Cancer Science |
巻: | 110 |
号: | 1 |
開始ページ: | 345 |
終了ページ: | 355 |
抄録: | Patient‐derived cancer organoid culture is an important live material that reflects clinical heterogeneity. However, the limited amount of organoids available for each case as well as the considerable amount of time and cost to expand in vitro makes it impractical to perform high‐throughput drug screening using organoid cultures from multiple patients. Here, we report an advanced system for the high‐throughput screening of 2427 drugs using the cancer tissue‐originated spheroid (CTOS) method. In this system, we apply the CTOS method in an ex vivo platform from xenograft tumors, using machines to handle CTOS and reagents, and testing a CTOS reference panel of multiple CTOS lines for the hit drugs. CTOS passages in xenograft tumors resulted in minimal changes of morphological and genomic status, and xenograft tumor generation efficiently expanded the number of CTOS to evaluate multiple drugs. Our panel of colorectal cancer CTOS lines exhibited diverse sensitivities to the hit compounds, demonstrating the usefulness of this system for investigating highly heterogeneous disease. |
著作権等: | © 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
URI: | http://hdl.handle.net/2433/236007 |
DOI(出版社版): | 10.1111/cas.13843 |
PubMed ID: | 30343529 |
出現コレクション: | 学術雑誌掲載論文等 |
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