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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| 978-1-4939-9740-4_16.pdf | 75.86 kB | Adobe PDF | 見る/開く |
| タイトル: | Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal |
| 著者: | Yamano, Takashi    https://orcid.org/0000-0001-6578-8095 (unconfirmed)Fukuzawa, Hideya |
| 著者名の別形: | 山野, 隆志 福澤, 秀哉 |
| キーワード: | Chlamydomonas reinhardtii Electroporation Photosynthesis Protist Square electric pulse |
| 発行日: | 2020 |
| 出版者: | Springer US |
| 誌名: | Methods in Molecular Biology |
| 巻: | 2050 |
| 開始ページ: | 155 |
| 終了ページ: | 161 |
| 抄録: | The green alga Chlamydomonas reinhardtii has been widely used to study many biological processes, including photosynthesis, flagellar motility, sexual reproduction, metabolism, and genetics. Here, we describe a step-by-step protocol of rapid and efficient transformation method for wild type cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to the transformation of other industrially useful algae including diatom by optimizing the electric conditions. |
| 記述: | Yamano T., Fukuzawa H. (2020) Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal. In: Li S., Chang L., Teissie J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 2050. Humana, New York, NY |
| 著作権等: | This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at: https://doi.org/10.1007/978-1-4939-9740-4_16. The full-text file will be made open to the public on 30 August 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
| URI: | http://hdl.handle.net/2433/244795 |
| DOI(出版社版): | 10.1007/978-1-4939-9740-4_16 |
| PubMed ID: | 31468489 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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