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タイトル: | Elucidation of the mechanism of subunit exchange in αB crystallin oligomers |
著者: | Inoue, Rintaro Sakamaki, Yusuke Takata, Takumi Wood, Kathleen Morishima, Ken https://orcid.org/0000-0003-1091-3850 (unconfirmed) Sato, Nobuhiro https://orcid.org/0000-0002-6960-6567 (unconfirmed) Okuda, Aya https://orcid.org/0000-0002-6222-8364 (unconfirmed) Shimizu, Masahiro Urade, Reiko https://orcid.org/0000-0002-9232-6176 (unconfirmed) Fujii, Noriko Sugiyama, Masaaki |
著者名の別形: | 井上, 倫太郎 酒巻, 裕介 髙田, 匠 守島, 健 佐藤, 信浩 奥田, 綾 清水, 将裕 裏出, 令子 藤井, 紀子 杉山, 正明 |
キーワード: | Biological techniques Biophysics |
発行日: | 28-Jan-2021 |
出版者: | Springer Nature |
誌名: | Scientific Reports |
巻: | 11 |
論文番号: | 2555 |
抄録: | AlphaB crystallin (αB-crystallin) is a key protein for maintaining the long-term transparency of the eye lens. In the eye lens, αB-crystallin is a “dynamical” oligomer regulated by subunit exchange between the oligomers. To elucidate the unsettled mechanism of subunit exchange in αB-crystallin oligomers, the study was carried out at two different protein concentrations, 28.5 mg/mL (dense sample) and 0.45 mg/mL (dilute sample), through inverse contrast matching small-angle neutron scattering. Interestingly, the exchange rate of the dense sample was the same as that of the dilute sample. From analytical ultracentrifuge measurements, the coexistence of small molecular weight components and oligomers was detected, regardless of the protein concentration. The model proposed that subunit exchange could proceed through the assistance of monomers and other small oligomers; the key mechanism is attaching/detaching monomers and other small oligomers to/from oligomers. Moreover, this model successfully reproduced the experimental results for both dense and dilute solutions. It is concluded that the monomer and other small oligomers attaching/detaching mainly regulates the subunit exchange in αB-crystallin oligomer. |
著作権等: | © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
URI: | http://hdl.handle.net/2433/261677 |
DOI(出版社版): | 10.1038/s41598-021-82250-z |
PubMed ID: | 33510404 |
出現コレクション: | 学術雑誌掲載論文等 |
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