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タイトル: | Rhodopsin-mediated light-off-induced protein kinase A activation in mouse rod photoreceptor cells |
著者: | Sato, Shinya Yamashita, Takahiro Matsuda, Michiyuki https://orcid.org/0000-0002-5876-9969 (unconfirmed) |
著者名の別形: | 佐藤, 慎哉 山下, 高廣 松田, 道行 |
キーワード: | retina two-photon microscopy FRET biosensor protein kinase A rhodopsin |
発行日: | Oct-2020 |
出版者: | National Academy of Sciences |
誌名: | Proceedings of the National Academy of Sciences of the United States of America |
巻: | 117 |
号: | 43 |
開始ページ: | 26996 |
終了ページ: | 27003 |
抄録: | Light-induced extrasynaptic dopamine release in the retina reduces adenosine 3′, 5′-cyclic monophosphate (cAMP) in rod photoreceptor cells, which is thought to mediate light-dependent desensitization. However, the fine time course of the cAMP dynamics in rods remains elusive due to technical difficulty. Here, we visualized the spatiotemporal regulation of cAMP-dependent protein kinase (PKA) in mouse rods by two-photon live imaging of retinal explants of PKAchu mice, which express a fluorescent biosensor for PKA. Unexpectedly, in addition to the light-on-induced suppression, we observed prominent light-off-induced PKA activation. This activation required photopic light intensity and was confined to the illuminated rods. The estimated maximum spectral sensitivity of 489 nm and loss of the light-off-induced PKA activation in rod-transducin-knockout retinas strongly suggest the involvement of rhodopsin. In support of this notion, rhodopsin-deficient retinal explants showed only the light-on-induced PKA suppression. Taken together, these results suggest that, upon photopic light stimulation, rhodopsin and dopamine signals are integrated to shape the light-off-induced cAMP production and following PKA activation. This may support the dark adaptation of rods. |
記述: | 網膜の知られざる光応答を顕微鏡観察で発見 --光センサー細胞が暗黒に反応した--. 京都大学プレスリリース. 2020-10-14. Detect with PKAchu. 京都大学プレスリリース. 2020-10-30. |
著作権等: | This is a postprint of the article, which has been published in final form at https://doi.org/10.1073/pnas.2009164117. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/264443 |
DOI(出版社版): | 10.1073/pnas.2009164117 |
PubMed ID: | 33046651 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research/research_results/2020/201013_2.html https://www.kyoto-u.ac.jp/en/research/research_results/2020/201013_2.html |
出現コレクション: | 学術雑誌掲載論文等 |
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