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タイトル: | A carlactonoic acid methyltransferase that contributes to the inhibition of shoot branching in Arabidopsis |
著者: | Mashiguchi, Kiyoshi https://orcid.org/0000-0002-7234-887X (unconfirmed) Seto, Yoshiya Onozuka, Yuta Suzuki, Sarina Takemoto, Kiyoko Wang, Yanting Dong, Lemeng Asami, Kei Noda, Ryota Kisugi, Takaya Kitaoka, Naoki Akiyama, Kohki Bouwmeester, Harro Yamaguchi, Shinjiro https://orcid.org/0000-0002-0786-1757 (unconfirmed) |
著者名の別形: | 増口, 潔 瀬戸, 義哉 小野塚, 祐太 鈴木, 紗里奈 竹本, 貴世子 浅見, 慶 野田, 涼太 来生, 貴也 北岡, 直樹 秋山, 康紀 山口, 信次郎 |
キーワード: | PLANT HORMONES BIOSYNTHESIS TERPENOID STRIGOLACTONE |
発行日: | Apr-2022 |
出版者: | Proceedings of the National Academy of Sciences |
誌名: | Proceedings of the National Academy of Sciences (PNAS) |
巻: | 119 |
号: | 14 |
論文番号: | e2111565119 |
抄録: | Strigolactones (SLs) are plant hormones that regulate shoot branching and diverse developmental processes. They are biosynthesized from carotenoid molecules via a key biosynthetic precursor called carlactone (CL) and its carboxylated analog, carlactonoic acid (CLA). We have previously identified the methyl esterified derivative of CLA, methyl carlactonoate (MeCLA), as an endogenous SL-like molecule in Arabidopsis. Neither CL nor CLA could interact with the receptor protein, Arabidopsis DWARF14 (AtD14), in vitro, while MeCLA could, suggesting that the methylation step of CLA is critical to convert a biologically inactive precursor to a bioactive compound in the shoot branching inhibition pathway. Here, we show that a member of the SABATH protein family (At4g36470) efficiently catalyzes methyl esterification of CLA using S-adenosyl-L-methionine (SAM) as a methyl donor. We named this enzyme CLAMT for CLA methyltransferase. The Arabidopsis loss-of-function clamt mutant accumulated CLA and had substantially reduced MeCLA content compared with wild type (WT), showing that CLAMT is the main enzyme that catalyzes CLA methylation in Arabidopsis. The clamt mutant displayed an increased branching phenotype, yet the branch number was less than that of severe SL biosynthetic mutants. Exogenously applied MeCLA, but not CLA, restored the branching phenotype of the clamt mutant. In addition, grafting experiments using the clamt and other SL biosynthetic mutants suggest that CL and CLA are transmissible from root to shoot. Taken together, our results demonstrate a significant role of CLAMT in the shoot branching inhibition pathway in Arabidopsis. |
記述: | 植物の枝分かれ調節ホルモンの合成メカニズムを解明 --植物ホルモンを活性化する酵素タンパク質を発見--. 京都大学プレスリリース. 2022-03-30. |
著作権等: | This is a postprint of the article, which has been published in final form at https://doi.org/10.1073/pnas.2111565119. The full-text file will be made open to the public on September 28, 2022 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/269149 |
DOI(出版社版): | 10.1073/pnas.2111565119 |
PubMed ID: | 35344437 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2022-03-30-0 |
出現コレクション: | 学術雑誌掲載論文等 |
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