Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/102139
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Type: Journal article
Title: Efficacy of low concentrations of sodium hypochlorite and low-powered Er,Cr: YSGG laser activated irrigation against an Enterococcus faecalis biofilm
Author: Christo, J.
Zilm, P.
Sullivan, T.
Cathro, P.
Citation: International Endodontic Journal, 2016; 49(3):279-286
Publisher: Wiley
Issue Date: 2016
ISSN: 0143-2885
1365-2591
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Responsibility: 
J. E. Christo, P. S. Zilm, T. Sullivan and P. R. Cathro
Abstract: Aim: To establish the antibacterial efficacy of low concentrations of sodium hypochlorite with and without Er,Cr:YSGG laser activation on Enterococcus faecalis biofilms in extracted teeth. Methodology: The root canals of 96 decoronated single-rooted extracted human teeth were prepared to a size 40, 0.06 taper 1 mm beyond the apex. They were mounted within a flow cell, which was sterilized before pumping a nutrient media through the root canals. The flow cell was inoculated with E. faecalis (ATCC 700802) and cultivated for 4 weeks. The rootends were sealed, and the roots were then subjected to one of six treatment groups: group 1: syringe irrigation (SI) with saline (control) using a 27 -gauge Monoject needle 1 mm from the apex for 2 min; group 2: as for group 1 but with 1% NaOCl; group 3: as for group 1 but with 4% NaOCl; group 4: 0.5% NaOCl irrigation for 15 s followed by laser-activated irrigation (LAI) with four 15-s cycles replenishing the irrigant between cycles; group 5: as for group 4 but with 1% NaOCl as the irrigant; group 6: as for group 4 but with 4% NaOCl as the irrigant. Following treatment, teeth were crushed and viable bacteria were quantitated by serial dilution and plating. The colonyforming unit values were compared between groups using one-way ANOVA and Tukey-adjusted post hoc tests. A two-tailed P value of <0.05 was considered statistically significant. Results: The mean number of cells recovered from the 1% NaOCl SI group was significantly higher than that from the 4% NaOCl LAI group (P = 0.02). Conclusion: Within the limitations of this laboratory study, low-powered (0.5 W) Er,Cr:YSGG laser activation did not improve the antibacterial effect of low concentrations of sodium hypochlorite.
Keywords: biofilm; endodontics; laser-activated irrigation; root canal disinfection; Sodium hypochlorite
Description: First published: 1 April 2015
Rights: © 2015 International Endodontic Journal. Published by John Wiley & Sons Ltd.
DOI: 10.1111/iej.12447
Published version: http://dx.doi.org/10.1111/iej.12447
Appears in Collections:Aurora harvest 7
Dentistry publications

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