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https://hdl.handle.net/2440/126296
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Type: | Journal article |
Title: | Prevalence of Arcobacter and other pathogenic bacteria in river water in Nepal |
Author: | Shrestha Ghaju, R. Tandukar, S. Sherchan, S.P. Bhandari, D. Tanaka, Y. Sherchand, J.B. Haramoto, E. |
Citation: | Water: an open access journal, 2019; 11(7):1416-1-1416-9 |
Publisher: | MDPI |
Issue Date: | 2019 |
ISSN: | 2073-4441 2073-4441 |
Statement of Responsibility: | Rajani Ghaju Shrestha, Sarmila Tandukar, Dinesh Bhandari, Samendra P. Sherchan, Yasuhiro Tanaka, Jeevan B. Sherchand and Eiji Haramoto |
Abstract: | This study aims to determine the diversity of pathogenic bacteria in the Bagmati River, Nepal, during a one-year period. A total of 18 river water samples were collected from three sites (n = 6 per site) along the river. Bacterial DNA, which were extracted from the water samples, were analyzed for bacterial 16S rRNA genes by next-generation sequencing for 13 of 18 samples, and by quantitative PCR targeting Arcobacter for all 18 samples. The 16S rRNA sequencing identified an average of 97,412 ± 35,909 sequences/sample, which were then categorized into 28 phyla, 61 classes, and 709 bacterial genera. Eighteen (16%) genera of 111 potential pathogenic bacteria were detected with abundance ratios of >1%; Arcobacter, Acinetobacter, and Prevotella were the dominant genera. The Arcobacter abundance ratios were 28.6% (n = 1), 31.3 ± 15.8% (n = 6), and 31.8 ± 17.2% (n = 6) at the upstream, midstream, and downstream sites, respectively. Arcobacter was detected in 14 (78%) of 18 samples tested, with concentrations ranging from 6.7 to 10.7 log10 copies/100 mL, based on quantitative PCR. Our results demonstrate the poor bacterial quality of the Bagmati River water, suggesting a need for implementing more measures to reduce fecal contamination in the river water. |
Keywords: | Arcobacter; next-generation sequencing; pathogenic bacteria; quantitative PCR; river water |
Description: | Published: 10 July 2019 |
Rights: | © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
DOI: | 10.3390/w11071416 |
Published version: | https://www.mdpi.com/2073-4441/11/7/1416 |
Appears in Collections: | Aurora harvest 4 Public Health publications |
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hdl_126296.pdf | Published version | 872.3 kB | Adobe PDF | View/Open |
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