Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/17414
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Increased intracellular pro- and anti-inflammatory cytokines in bronchoalveolar lavage T cells of stable lung transplant patients |
Author: | Hodge, G. Hodge, S. Reynolds, P. Holmes, M. |
Citation: | Transplantation, 2005; 80(8):1040-1045 |
Publisher: | Lippincott Williams & Wilkins |
Issue Date: | 2005 |
ISSN: | 0041-1337 1534-6080 |
Abstract: | <h4>Background</h4>Allograft rejection remains a major cause of morbidity and mortality following lung transplantation and is associated with an increased expression of T-cell proinflammatory cytokines. We have recently shown that peripheral blood T-cell proinflammatory cytokine production was significantly reduced in stable lung transplant patients consistent with immunosuppression therapy. However, analysis of inflammatory cytokine profiles in bronchoalveolar lavage (BAL) T cells may be more relevant than peripheral blood T cells for assessing graft status.<h4>Methods</h4>To investigate the immunomodulatory effects of currently used immunosuppressive regimens on BAL T-cell cytokine production, whole blood and BAL from stable lung transplant patients and control volunteers was stimulated in vitro and cytokine production by CD8+ and CD4+ T-cell subsets determined using multiparameter flow cytometry.<h4>Results</h4>There was a significant decrease in T-cell proinflammatory cytokine production in BAL compared with blood from control subjects but not transplant patients. Anti-inflammatory cytokine IL-4 was increased in BAL compared with blood from both groups. There was a significant increase in IFNgamma, IL-2, IL-4, TGFbeta, and TNFalpha production by CD8 T cells and IFNgamma and TNFalpha production by CD4 T cells in BAL from transplant patients compared with controls.<h4>Conclusions</h4>We have shown decreased T-cell pro- and anti-inflammatory cytokine production in BAL compared with blood in control subjects but not in stable lung transplant patients. Current immunosuppression protocols have limited effect on T-cell proinflammatory cytokine production in BAL but do upregulate anti-inflammatory cytokines IL-4 and TGFbeta. Drugs that effectively reduce T-cell proinflammatory cytokine production in BAL may improve current protocols for reducing graft rejection in these patients. |
Keywords: | Lung CD4-Positive T-Lymphocytes CD8-Positive T-Lymphocytes Bronchoalveolar Lavage Fluid Humans Immunosuppressive Agents Cytokines CD4-CD8 Ratio Lung Transplantation Graft Rejection Adult Female Male |
DOI: | 10.1097/01.TP.0000173997.92753.25 |
Published version: | http://dx.doi.org/10.1097/01.tp.0000173997.92753.25 |
Appears in Collections: | Aurora harvest 6 Paediatrics publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.