Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/23278
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Type: Journal article
Title: Etanercept decreases tumor necrosis factor-alpha activity in chronic wound fluid
Author: Cowin, A.
Hatzirodos, N.
Rigden, J.
Fitridge, R.
Belford, D.
Citation: Wound Repair and Regeneration, 2006; 14(4):421-426
Publisher: Blackwell Publishing Inc
Issue Date: 2006
ISSN: 1067-1927
1524-475X
Statement of
Responsibility: 
Allison J. Cowin, Nicholas Hatzirodos, Justin Rigden, Robert Fitridge and David A. Belford
Abstract: High levels of tumor necrosis factor-α (TNF-α), a pro-inflammatory cytokine, are present in the wound fluid of chronic nonhealing wounds. This leads to increased inflammation, cytokine expression, and ultimately results in impaired wound healing and tissue destruction. Etanercept is a recombinant fusion protein that consists of the soluble TNF receptor (p75) linked to the Fc portion of human IgG1. It is an effective inhibitor of TNF-α and has been shown to provide rapid and sustained improvement in rheumatoid arthritis by acting as a soluble receptor binding TNF-α and preventing its proinflammatory activities. Therefore, the aim of this study was to determine whether Etanercept could inhibit TNF-α activity in chronic wound fluid. Wound fluid was collected from the venous leg ulcers of 16 different patients. The effect of Etanercept on TNF-α activity was evaluated using both a TNF-α bioassay and an enzyme-linked immunoassay. Etanercept was found to reduce the cytotoxic effect of chronic wound fluid on L929 fibroblasts by approximately 30% and neutralized TNF-α binding in the enzyme-linked immunoassay by up to 80%. Direct application of Etanercept to chronic wounds may therefore reduce the inflammatory activity of TNF-α, which could reduce the chronicity of venous leg ulcers and thus aid in the healing of these wounds.
Keywords: Fibroblasts
Humans
Varicose Ulcer
Chronic Disease
Tumor Necrosis Factor-alpha
Immunoglobulin G
Receptors, Tumor Necrosis Factor
Immunologic Factors
Enzyme-Linked Immunosorbent Assay
Biological Assay
Cell Culture Techniques
Aged
Aged, 80 and over
Middle Aged
Etanercept
Description: The definitive version is available at www.blackwell-synergy.com
DOI: 10.1111/j.1743-6109.2006.00141.x
Published version: http://dx.doi.org/10.1111/j.1743-6109.2006.00141.x
Appears in Collections:Aurora harvest 2
Paediatrics publications

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