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https://hdl.handle.net/2440/28150
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Type: | Journal article |
Title: | Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa |
Author: | Paton, A. Paton, J. |
Citation: | Journal of Clinical Microbiology, 2002; 40(1):271-274 |
Publisher: | Amer Soc Microbiology |
Issue Date: | 2002 |
ISSN: | 0095-1137 1098-660X |
Statement of Responsibility: | Adrienne W. Paton and James C. Paton |
Abstract: | We recently described a novel megaplasmid-encoded adhesin produced by certain Shiga toxigenic Escherichia coli (STEC) strains that lack the locus for enterocyte effacement (LEE) pathogenicity island. This adhesin, designated Saa (STEC autoagglutinating adhesin), may be a marker for a subset of LEE-negative STEC strains capable of causing severe gastrointestinal and systemic diseases in humans. In this study, we developed a pentavalent PCR assay for the detection of saa as well as other proven and putative STEC virulence genes (stx1, stx2, eae, and ehxA). The five primer pairs used in the assay do not interfere with each other and generate amplification products of 119, 180, 255, 384, and 534 bp. |
Keywords: | Feces Humans Escherichia coli Escherichia coli Infections Adhesins, Bacterial Escherichia coli Proteins DNA Primers Shiga Toxins Sensitivity and Specificity Polymerase Chain Reaction Virulence |
Description: | Copyright © 2002, American Society for Microbiology. All Rights Reserved. |
DOI: | 10.1128/JCM.40.1.271-274.2002 |
Published version: | http://jcm.asm.org/cgi/content/abstract/40/1/271 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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