Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/34800
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Know thy fly |
Author: | O'Keefe, L. Smibert, P. Colella, A. Chataway, T. Saint, R. Richards, R. |
Citation: | Trends in Genetics, 2007; 23(5):238-242 |
Publisher: | Elsevier Science London |
Issue Date: | 2007 |
ISSN: | 0168-9525 |
Organisation: | Centre for the Molecular Genetics of Development |
Statement of Responsibility: | Louise V. O’Keefe, Peter Smibert, Alex Colella, Tim K. Chataway, Robert Saint, and Robert I. Richards |
Abstract: | The generation and analysis of mutants is central to studies of gene function in model organisms. Methods for random mutagenesis in Drosophila melanogaster have been available for many years, but an alternative approach--targeted mutagenesis using homologous recombination--has only recently been developed. This approach has the advantage of specificity, because genes of interest can be altered. One might expect with a gene-targeting approach that the frequency of background mutations would be minimal. Unfortunately, we have found that this is not the case. Although the possibility of background mutations arising during homologous-recombination-based gene targeting has been raised in the literature, it is not routinely taken into account when using this technique. Our experience suggests that it can be a considerable problem but that it has a relatively simple solution. |
Keywords: | Animals Drosophila melanogaster Gene Targeting Proteomics Mutagenesis Recombination, Genetic Mutation Alleles Genes, Insect Models, Genetic |
DOI: | 10.1016/j.tig.2007.03.007 |
Published version: | http://dx.doi.org/10.1016/j.tig.2007.03.007 |
Appears in Collections: | Aurora harvest 6 Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.