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https://hdl.handle.net/2440/37471
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Type: | Journal article |
Title: | Decreased expression of tumor necrosis factor-α-stimulated gene 6 in cumulus cells of the cyclooxygenase-2 and EP2 null mice |
Other Titles: | Decreased expression of tumor necrosis factor-alpha-stimulated gene 6 in cumulus cells of the cyclooxygenase-2 and EP2 null mice |
Author: | Ochsner, S. Russell, D. Day, A. Breyer, R. Richards, J. |
Citation: | Endocrinology, 2003; 144(3):1008-1019 |
Publisher: | Endocrine Soc |
Issue Date: | 2003 |
ISSN: | 0013-7227 1945-7170 |
Statement of Responsibility: | Scott A. Ochsner, Darryl L. Russell, Anthony J. Day, Richard M. Breyer and Joanne S. Richards |
Abstract: | Ovulation, the release of fertilizable oocytes from mature follicles, involves tissue remodeling and increased prostaglandin (PG) signaling. Cyclooxygenase (COX)-2 is the rate-limiting enzyme during PG synthesis. Female mice null for either COX-2 or the PGE2 receptor EP2 are infertile, show decreased ovulation, and exhibit abnormal cumulus expansion. Cumulus expansion is the production of a complex extracellular matrix surrounding the cumulus-oocyte complex (COC). Matrix components consist of hyaluronan, proteoglycans, and proteins with hyaluronan binding domains. One such hyaluronan binding protein is TNF-stimulated gene 6 (TSG-6). By various methods, we show induction of TSG-6 and hyaluronan synthase-2 mRNA in ovaries of mice treated with pregnant mare serum gonadotropin and human chorionic gonadotropin. By in situ hybridization, we show that both genes are expressed in periantral mural granulosa cells and cumulus cells of the mouse ovary. Notably, RT-PCR and in situ hybridization show that TSG-6 mRNA but not hyaluronan synthase-2 mRNA expression is selectively reduced in cumulus cells of COX-2 and EP2 null mice. Western analysis further confirms that TSG-6 protein is reduced in isolated COCs but remains covalently associated with inter-trypsin inhibitor in COX-2 null mice. These observations identify TSG-6 as a target of PG action and show that its production in ovulatory follicles is associated with proper formation of the cumulus-derived extracellular matrix. |
Keywords: | Ovarian Follicle Animals Mice, Inbred C57BL Mice, Knockout Mice Isoenzymes Glucuronosyltransferase Alpha-Globulins Cell Adhesion Molecules Receptors, Prostaglandin E RNA, Messenger Blotting, Western In Situ Hybridization Reverse Transcriptase Polymerase Chain Reaction Gene Expression Female Prostaglandin-Endoperoxide Synthases Cyclooxygenase 2 Receptors, Prostaglandin E, EP2 Subtype Hyaluronan Synthases |
Rights: | © 2003 by The Endocrine Society |
DOI: | 10.1210/en.2002-220435 |
Published version: | http://dx.doi.org/10.1210/en.2002-220435 |
Appears in Collections: | Aurora harvest 6 Obstetrics and Gynaecology publications |
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