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https://hdl.handle.net/2440/43410
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Type: | Journal article |
Title: | Low CD4 T cell immunity to pneumolysin is associated with nasopharyngeal carriage of pneumococci in children |
Author: | Zhang, Q. Bagrade, L. Bernatoniene, J. Clarke, E. Paton, J. Mitchell, T. Nunez, D. Finn, A. |
Citation: | Journal of Infectious Diseases, 2007; 195(8):1194-1202 |
Publisher: | Univ Chicago Press |
Issue Date: | 2007 |
ISSN: | 0022-1899 1537-6613 |
Statement of Responsibility: | Qibo Zhang, Linda Bagrade, Jolanta Bernatoniene, Ed Clarke, James C. Paton, Tim J. Mitchell, Desmond A. Nunez, and Adam Finn |
Abstract: | <h4>Background</h4>Recent studies in mice have suggested that T cell immunity may be protective against pneumococcal infection.<h4>Methods</h4>CD4 T cell proliferative responses to the pneumococcal proteins pneumolysin (Ply), Ply toxoid (F433), and choline-binding protein A were investigated in peripheral blood mononuclear cells (PBMCs) and adenoidal mononuclear cells (MNCs) obtained from children undergoing adenoidectomy.<h4>Results</h4>Ply and F433 induce significant proliferation of CD4 T cells in both PBMCs and adenoidal MNCs, and both memory and naive phenotypes of CD4 T cells proliferated after stimulation. In PBMCs, CD4 T cell proliferation induced by Ply and F433, which was associated with increased production of interferon (IFN)- gamma and tumor necrosis factor (TNF)- alpha , was significantly lower in children who were culture positive for pneumococcus than in those who were culture negative for pneumococcus (P<.05). Between groups, no such difference was observed in adenoidal MNC CD4 T cell proliferation, which was associated with production of IFN- gamma and interleukin (IL)-10. The CD4 T cell proliferation induced by Ply and F433 was inhibited by antibodies to Toll-like receptor 4.<h4>Conclusion</h4>These data suggest that Ply induces CD4 T cell proliferative responses with production of IFN- gamma and TNF- alpha in PBMCs or of IFN- gamma and IL-10 in adenoidal MNCs, which may be important in modulating pneumococcal carriage in children. |
Keywords: | Nasopharynx CD4-Positive T-Lymphocytes Cells, Cultured Humans Streptococcus pneumoniae Pneumococcal Infections Tumor Necrosis Factor-alpha Bacterial Proteins Streptolysins Antibodies, Bacterial Carrier State Cell Proliferation Time Factors Child Child, Preschool Female Male Toll-Like Receptors Interferon-gamma |
Description: | © 2007 by the Infectious Diseases Society of America. All rights reserved. |
DOI: | 10.1086/512617 |
Published version: | http://www.journals.uchicago.edu/doi/abs/10.1086/512617 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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