Effect of solution environment on the purification of pertussis toxin

Wu, T.; Huang, Y.; Bi, J.; Zhang, Y.; Sun, L.; Sun, C.; Su, Z.

Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/51757

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Type:	Journal article
Title:	Effect of solution environment on the purification of pertussis toxin
Author:	Wu, T. Huang, Y. Bi, J. Zhang, Y. Sun, L. Sun, C. Su, Z.
Citation:	Shengwu Gongcheng Xuebao, 2008; 24(7):1279-1284
Publisher:	Allerton Press, Inc.
Issue Date:	2008
ISSN:	1042-749X 1872-2075
Statement of Responsibility:	Tie Wu, Yongdong Huang, Jingxiu Bi, Yan Zhang, Lijing Sun, Chunbao Sun and Zhiguo Su
Abstract:	The low recovery of pertussis toxin (PT) and the low resolving efficiency of chromatography, due to the instability of PT in low salt condition, are the main challenges for its purification. We aplied 2 mol/L urea to prevent the aggregation and disassociation of PT during the purification by ion-exchange chromatography (IEC) and gel filtration chromatography (GFC). The effect of urea on the purification of PT was studied by ELISA assay and non-reduced SDS-PAGE. The activity recoveries of PT and filamentous hemagglutinin (FHA) in IEC and GFC, the resolution efficiency in GFC and the purities of PT and FHA were improved obviously by adding 2 mol/L urea in the mobile phase. The results highlight the potential application of urea in the acellular pertussis vaccine (APV) manufacture procedure.
Keywords:	Humans Urea Pertussis Toxin Adhesins, Bacterial Virulence Factors, Bordetella Pertussis Vaccine Vaccines, Acellular Solutions Chromatography, Ion Exchange
DOI:	10.1016/S1872-2075(08)60061-8
Published version:	http://dx.doi.org/10.1016/s1872-2075(08)60061-8
Appears in Collections:	Aurora harvest 5 Chemical Engineering publications

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