Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/53662
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Type: Journal article
Title: The Effect of the JNK Inhibitor, JIP Peptide, on Human T Lymphocyte Proliferation and Cytokine Production
Author: Melino, M.
Hii, C.
McColl, S.
Ferrante, A.
Citation: Journal of Immunology, 2008; 181(10):7300-7306
Publisher: Amer Assoc Immunologists
Issue Date: 2008
ISSN: 0022-1767
1550-6606
Statement of
Responsibility: 
Michelle Melino, Charles S. Hii, Shaun R. McColl and Antonio Ferrante
Abstract: Although JNK is a potential target for treating chronic inflammatory diseases, its role in T lymphocyte function remains controversial. To overcome some of the previous limitations in addressing this issue we have used the recently described transactivator of transcription-JNK-interacting protein (TAT-JIP) peptide, a specific inhibitor that was derived from the minimal JNK-binding region of the scaffold protein, JNK-interacting protein 1 (JIP-1), coupled to the short cell-permeable HIV TAT sequence. Pretreatment of purified human T lymphocytes with the TAT-JIP peptide inhibited the phosphorylation of endogenous jun activated by PHA-PMA. This was associated with a corresponding inhibition of lymphoproliferation, and of IL-2, IFN-gamma, lymphotoxin, and IL-10 cytokine production. Similar results were also found using mouse splenic T cells. Examination of the specificity of TAT-JIP revealed that although the peptide was more selective than the pharmacological inhibitor, SP600125, it also inhibited cyclin-dependent kinase 2, p70 ribosomal protein S6 kinase, and serum and glucocorticoid-regulated kinase activity. Nevertheless, these data demonstrate for the first time the ability of the TAT-JIP peptide to inhibit the JNK pathway and the phosphorylation of jun in intact cells, thereby preventing the activation of the transcription factor, AP-1, and the production of Th1 and Th2 cytokines. Thus JNK could potentially be a target for the development of drugs for the treatment of autoimmune inflammatory diseases.
Keywords: T-Lymphocytes
Cells, Cultured
Animals
Humans
Mice
MAP Kinase Kinase 4
Adaptor Proteins, Signal Transducing
Peptide Fragments
Cytokines
Blotting, Western
Lymphocyte Activation
Cell Proliferation
Enzyme Activation
Phosphorylation
tat Gene Products, Human Immunodeficiency Virus
DOI: 10.4049/jimmunol.181.10.7300
Published version: http://www.jimmunol.org/cgi/content/full/181/10/7300
Appears in Collections:Aurora harvest 5
Paediatrics publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.