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https://hdl.handle.net/2440/56244
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Type: | Journal article |
Title: | CFD simulation coupled with population balance equations for aerated stirred bioreactors |
Author: | Zhang, H. Zhang, K. Fan, S. |
Citation: | Engineering in Life Sciences, 2009; 9(6):421-430 |
Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA |
Issue Date: | 2009 |
ISSN: | 1618-0240 1618-2863 |
Statement of Responsibility: | Hu Zhang, Kai Zhang and Shengdi Fan |
Abstract: | Mammalian cells have been widely used to produce therapeutic proteins in stirred bioreactors in suspension culture. Local hydrodynamics can have a great impact on cell proliferation and protein synthesis, but there are few reports on spatial heterogeneity of nutrients, gas bubbles, and mass transfer coefficients. We have employed computational fluid dynamics (CFD) coupled with population balance equations to study local hydrodynamics in a 20 L stirred bioreactor. The flow patterns, energy dissipation rates, gas volume fraction, gas bubble size distribution and local mass transfer coefficient have been displayed throughout the whole bioreactor. Their implications for mammalian cell culture have been discussed. This study provides an insight into rational design and optimum operation conditions in a stirred bioreactor for mammalian cell cultivation. |
Keywords: | CFD Gas-liquid flow Mammalian cell culture Stirred bioreactor |
DOI: | 10.1002/elsc.200800074 |
Published version: | http://dx.doi.org/10.1002/elsc.200800074 |
Appears in Collections: | Aurora harvest Chemical Engineering publications |
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