Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/57490
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Cross-presentation of male seminal fluid antigens elicits T cell activation to initiate the female immune response to pregnancy |
Author: | Moldenhauer, L. Diener, K. Thring, D. Brown, M. Hayball, J. Robertson, S. |
Citation: | Journal of Immunology, 2009; 182(12):8080-8093 |
Publisher: | Amer Assoc Immunologists |
Issue Date: | 2009 |
ISSN: | 0022-1767 1550-6606 |
Statement of Responsibility: | Lachlan M. Moldenhauer, Kerrilyn R. Diener, Dougal M. Thring, Michael P. Brown, John D. Hayball, and Sarah A. Robertson |
Abstract: | The events that generate T cell-mediated immune tolerance in early pregnancy are ill-defined. To investigate the significance of seminal fluid Ags in activating maternal T cells, and define the underlying Ag presentation pathways, OVA-specific T cells were adoptively transferred to female mice inseminated by males ubiquitously expressing membrane-bound OVA. OVA-reactive CD8_ OT-I and CD4_ OT-II T cells transferred to mated recipients expressed activation markers CD25 and CD69 and proliferated vigorously in the para-aortic lymph nodes, but not in distal lymph nodes or spleen, and OT-I T cells expressed IFN-_ and IL-2. In contrast, OT-I T cells transferred later in pregnancy or up to 10 days postpartum expressed CD25 and CD69 and proliferated in all peripheral lymphoid tissues examined. OVA Ag was present predominantly in the plasma fraction of seminal fluid, and seminal plasma, but not sperm, was necessary for T cell proliferation. Female H-2Kb bone marrow-derived cells expressing TAP were essential for OT-I T cell proliferation, but responses were not elicited by OVA Ag presented by paternal MHC in seminal fluid or associated with placental cells. This study shows that at conception, seminal fluid drives activation and expansion of paternal Ag-reactive CD4_ and CD8_ T cell populations, and female APCs have an essential role in cross-presenting Ag to CD8_ T cells via a TAP-dependent pathway. Delivery of paternal Ags and immune-deviating cytokines by seminal fluid at conception may activate Ag-dependent CD4_ and CD8_ regulatory T cells mediating tolerance of pregnancy. |
Keywords: | Antigen-Presenting Cells T-Lymphocytes Cells, Cultured Semen Bone Marrow Animals Mice, Knockout Mice Antigens Cytokines Lymphocyte Activation Cell Differentiation Cell Proliferation Cross-Priming Pregnancy Kinetics Female Male Sexual Behavior, Animal Immunity, Innate |
DOI: | 10.4049/jimmunol.0804018 |
Published version: | http://dx.doi.org/10.4049/jimmunol.0804018 |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.