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https://hdl.handle.net/2440/72990
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Type: | Journal article |
Title: | A two-staged model of Na+ exclusion in rice explained by 3D modeling of HKT transporters and alternative splicing |
Author: | Cotsaftis, O. Plett, D. Shirley, N. Tester, M. Hrmova, M. |
Citation: | PLoS One, 2012; 7(7):1-10 |
Publisher: | Public Library of Science |
Issue Date: | 2012 |
ISSN: | 1932-6203 1932-6203 |
Editor: | Baxter, I. |
Statement of Responsibility: | Olivier Cotsaftis, Darren Plett, Neil Shirley, Mark Tester and Maria Hrmova |
Abstract: | The HKT family of Na+ and Na+/K+ transporters is implicated in plant salinity tolerance. Amongst these transporters, the cereal HKT1;4 and HKT1;5 are responsible for Na+ exclusion from photosynthetic tissues, a key mechanism for plant salinity tolerance. It has been suggested that Na+ is retrieved from the xylem transpiration stream either in the root or the leaf sheath, protecting the leaf blades from excessive Na+ accumulation. However, direct evidence for this scenario is scarce. Comparative modeling and evaluation of rice (Oryza sativa) HKT-transporters based on the recent crystal structure of the bacterial TrkH K+ transporter allowed to reconcile transcriptomic and physiological data. For OsHKT1;5, both transcript abundance and protein structural features within the selectivity filter could control shoot Na+ accumulation in a range of rice varieties. For OsHKT1;4, alternative splicing of transcript and the anatomical complexity of the sheath needed to be taken into account. Thus, Na+ accumulation in a specific leaf blade seems to be regulated by abundance of a correctly spliced OsHKT1;4 transcript in a corresponding sheath. Overall, allelic variation of leaf blade Na+ accumulation can be explained by a complex interplay of gene transcription, alternative splicing and protein structure. |
Keywords: | Plant Leaves Plant Shoots Plant Roots Potassium Sodium Cation Transport Proteins Symporters Plant Proteins Protein Isoforms RNA, Messenger Crystallography, X-Ray Gene Expression Regulation, Plant Alternative Splicing Ion Transport Protein Conformation Alleles Models, Molecular Salinity Salt-Tolerant Plants Oryza |
Rights: | Copyright: © 2012 Cotsaftis et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pone.0039865 |
Grant ID: | ARC |
Published version: | http://dx.doi.org/10.1371/journal.pone.0039865 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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hdl_72990.pdf | Published version | 1.43 MB | Adobe PDF | View/Open |
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