Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/74744
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Type: | Journal article |
Title: | Absence of O antigen suppresses Shigella flexneri IcsA autochaperone region mutations |
Author: | Teh, M. Tran, N. Morona, R. |
Citation: | Microbiology, 2012; 158(11):2835-2850 |
Publisher: | Soc General Microbiology |
Issue Date: | 2012 |
ISSN: | 1350-0872 1465-2080 |
Statement of Responsibility: | Min Yan Teh, Elizabeth Ngoc Hoa Tran and Renato Morona |
Abstract: | The Shigella flexneri IcsA (VirG) protein is a polarly distributed autotransporter protein. IcsA functions as a virulence factor by interacting with the host actin regulatory protein N-WASP, which in turn activates the Arp2/3 complex, initiating actin polymerization. Formation of F-actin comet tails allows bacterial cell-to-cell spreading. Although various accessory proteins such as periplasmic chaperones and the β-barrel assembly machine (BAM) complex have been shown to be involved in the export of IcsA, the IcsA translocation mechanism remains to be fully elucidated. A putative autochaperone (AC) region (amino acids 634–735) located at the C-terminal end of the IcsA passenger domain, which forms part of the self-associating autotransporter (SAAT) domain, has been suggested to be required for IcsA biogenesis, as well as for N-WASP recruitment, based on mutagenesis studies. IcsAi proteins with linker insertion mutations within the AC region have a significant reduction in production and are defective in N-WASP recruitment when expressed in smooth LPS (S-LPS) S. flexneri. In this study, we have found that the LPS O antigen plays a role in IcsAi production based on the use of an rmlD (rfbD) mutant having rough LPS (R-LPS) and a novel assay in which O antigen is depleted using tunicamycin treatment and then regenerated. In addition, we have identified a new N-WASP binding/interaction site within the IcsA AC region. |
Keywords: | Hela Cells Humans Shigella flexneri Dysentery, Bacillary O Antigens Bacterial Proteins DNA-Binding Proteins Transcription Factors Down-Regulation Amino Acid Motifs Protein Binding Protein Transport Mutation Wiskott-Aldrich Syndrome Protein, Neuronal Promoter Regions, Genetic |
Rights: | © 2012 SGM |
DOI: | 10.1099/mic.0.062471-0 |
Published version: | http://dx.doi.org/10.1099/mic.0.062471-0 |
Appears in Collections: | Aurora harvest 7 Molecular and Biomedical Science publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_74744.pdf | Accepted version | 19 MB | Adobe PDF | View/Open |
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