Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/81165
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: One-step cloning and chromosomal integration of DNA
Author: St-Pierre, F.
Cui, L.
Priest, D.
Endy, D.
Dodd, I.
Shearwin, K.
Citation: ACS Synthetic Biology, 2013; 2(9):537-541
Publisher: American Chemical Society
Issue Date: 2013
ISSN: 2161-5063
2161-5063
Statement of
Responsibility: 
François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin
Abstract: We describe "clonetegration", a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.
Keywords: Chromosomes, Bacterial
Escherichia coli
Salmonella typhimurium
DNA
DNA Primers
Cloning, Molecular
Genetic Engineering
Transformation, Genetic
Genetic Vectors
Rights: Copyright © 2013 American Chemical Society
DOI: 10.1021/sb400021j
Grant ID: http://purl.org/au-research/grants/arc/DP110101470
http://purl.org/au-research/grants/arc/DP110100824
Published version: http://dx.doi.org/10.1021/sb400021j
Appears in Collections:Aurora harvest 4
Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.