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https://hdl.handle.net/2440/81165
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Type: | Journal article |
Title: | One-step cloning and chromosomal integration of DNA |
Author: | St-Pierre, F. Cui, L. Priest, D. Endy, D. Dodd, I. Shearwin, K. |
Citation: | ACS Synthetic Biology, 2013; 2(9):537-541 |
Publisher: | American Chemical Society |
Issue Date: | 2013 |
ISSN: | 2161-5063 2161-5063 |
Statement of Responsibility: | François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin |
Abstract: | We describe "clonetegration", a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids. |
Keywords: | Chromosomes, Bacterial Escherichia coli Salmonella typhimurium DNA DNA Primers Cloning, Molecular Genetic Engineering Transformation, Genetic Genetic Vectors |
Rights: | Copyright © 2013 American Chemical Society |
DOI: | 10.1021/sb400021j |
Grant ID: | http://purl.org/au-research/grants/arc/DP110101470 http://purl.org/au-research/grants/arc/DP110100824 |
Published version: | http://dx.doi.org/10.1021/sb400021j |
Appears in Collections: | Aurora harvest 4 Molecular and Biomedical Science publications |
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