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https://hdl.handle.net/2440/82216
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Type: | Journal article |
Title: | Induction of apoptosis by the medium-chain length fatty acid lauric acid in colon cancer cells due to induction of oxidative stress |
Author: | Fauser, J. Matthews, G. Cummins, A. Howarth, G. |
Citation: | Chemotherapy: international journal of experimental and clinical chemotherapy, 2014; 59(3):214-224 |
Publisher: | Karger |
Issue Date: | 2014 |
ISSN: | 0009-3157 1421-9794 |
Statement of Responsibility: | J.K. Fauser, G.M. Matthews, A.G. Cummins, G.S. Howarth |
Abstract: | <h4>Background</h4>Fatty acids are classified as short-chain (SCFA), medium-chain (MCFA) or long-chain and hold promise as adjunctive chemotherapeutic agents for the treatment of colorectal cancer. The antineoplastic potential of MCFA remains underexplored; accordingly, we compared the MCFA lauric acid (C12:0) to the SCFA butyrate (C4:0) in terms of their capacity to induce apoptosis, modify glutathione (GSH) levels, generate reactive oxygen species (ROS), and modify phases of the cell cycle in Caco-2 and IEC-6 intestinal cell lines.<h4>Methods</h4>Caco-2 and IEC-6 cells were treated with lauric acid, butyrate, or vehicle controls. Apoptosis, ROS, and cell cycle analysis were determined by flow cytometry. GSH availability was assessed by enzymology.<h4>Results</h4>Lauric acid induced apoptosis in Caco-2 (p < 0.05) and IEC-6 cells (p < 0.05) compared to butyrate. In Caco-2 cells, lauric acid reduced GSH availability and generated ROS compared to butyrate (p < 0.05). Lauric acid reduced Caco-2 and IEC-6 cells in G0/G1and arrested cells in the S and G2/M phases. Lauric acid induced apoptosis in IEC-6 cells compared to butyrate (p < 0.05). Butyrate protected IEC-6 cells from ROS-induced damage, whereas lauric acid induced high levels of ROS compared to butyrate.<h4>Conclusion</h4>Compared to butyrate, lauric acid displayed preferential antineoplastic properties, including induction of apoptosis in a CRC cell line. |
Keywords: | Medium-chain fatty acid Short-chain fatty acid Colorectal cancer Caco-2 IEC-6 Apoptosis Redox Cell cycle Glutathione Reactive oxygen species |
Rights: | © 2013 S. Karger AG, Basel |
DOI: | 10.1159/000356067 |
Published version: | http://dx.doi.org/10.1159/000356067 |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest 4 |
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