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https://hdl.handle.net/2440/89365
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Type: | Journal article |
Title: | Progress in understanding the assembly process of bacterial O-antigen |
Author: | Kalynych, S. Morona, R. Cygler, M. |
Citation: | FEMS Microbiology Reviews, 2014; 38(5):1048-1065 |
Publisher: | Wiley |
Issue Date: | 2014 |
ISSN: | 1574-6976 1574-6976 |
Statement of Responsibility: | Sergei Kalynych, Renato Morona & Miroslaw Cygler |
Abstract: | The discovery that the surfaces of Gram-negative bacteria often carry unique polysaccharide signatures pre-dates most seminal discoveries of molecular biology and biochemistry of the 20th century. The O-antigen component of the lipopolysaccharide has been one of the most intensely studied bacterial polysaccharide surface structures for over 80 years. Yet, many questions about the mechanism of biosynthesis of the O-antigen and its transport to the cell surface remain unanswered. In this review we provide an overview of how the molecular basis of the O-antigen assembly and trafficking were unraveled in a historical context. We pay particular attention to the emergence of novel technological approaches and how they fueled the elucidation of the O-antigen maturation process. Moreover, we provide a brief perspective on the biosynthesis of enterobacterial common antigen and underline the similarities and differences between the pathways used to assemble these two surface polysaccharides. Finally, we highlight key discoveries that led to the understanding of the mechanistic basis of bacteriophage-induced O-antigen modifications. We place special emphasis on the regulation of the length of O-antigen polymers and provide a detailed overview of the models explaining the O-antigen length determination. Finally, we highlight outstanding questions that need to be addressed both structurally and functionally to advance our understanding of the O-antigen assembly, trafficking and export within cellular and molecular contexts. |
Keywords: | O-antigen biosynthesis O-antigen genetics O-antigen length determination O-antigen transport gram-negative bacterial surface surface carbohydrates |
Rights: | © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved. |
DOI: | 10.1111/1574-6976.12070 |
Published version: | http://dx.doi.org/10.1111/1574-6976.12070 |
Appears in Collections: | Aurora harvest 7 Microbiology and Immunology publications |
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hdl_89365.pdf | Accepted version | 1.97 MB | Adobe PDF | View/Open |
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