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https://hdl.handle.net/2445/24655
Title: | Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway |
Author: | Sarri Plans, Elisabet Martínez Rodríguez, Susana Eva Jiménez, Nuria Claro Izaguirre, Enrique Mérida, Isabel Burger, Koert N. J. Egea Guri, Gustavo Fernández Ulibarri, Inés Vilella, Montserrat Lázaro Diégez, Francisco |
Keywords: | Aparell de Golgi Reticle endoplasmàtic Proteïnes Citologia Transport biològic Golgi apparatus Endoplasmic reticulum Proteins Cytology Biological transport |
Issue Date: | 2007 |
Publisher: | American Society for Cell Biology |
Abstract: | Diacylglycerol is necessary for trans-Golgi network (TGN) to cell surface transport, but its functional relevance in the early secretory pathway is unclear. Although depletion of diacylglycerol did not affect ER-to-Golgi transport, it led to a redistribution of the KDEL receptor to the Golgi, indicating that Golgi-to-ER transport was perturbed. Electron microscopy revealed an accumulation of COPI-coated membrane profiles close to the Golgi cisternae. Electron tomography showed that the majority of these membrane profiles originate from coated buds, indicating a block in membrane fission. Under these conditions the Golgi-associated pool of ARFGAP1 was reduced, but there was no effect on the binding of coatomer or the membrane fission protein CtBP3/BARS to the Golgi. The addition of 1,2-dioctanoyl-sn-glycerol or the diacylglycerol analogue phorbol 12,13-dibutyrate reversed the effects of endogenous diacylglycerol depletion. Our findings implicate diacylglycerol in the retrograde transport of proteins from Golgi to the ER and suggest that it plays a critical role at a late stage of COPI vesicle formation. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E07-04-0334 |
It is part of: | Molecular Biology of the Cell, 2007, vol. 18, núm. 9, p. 3250-3263 |
URI: | https://hdl.handle.net/2445/24655 |
DOI: | http://dx.doi.org/10.1091/mbc.E07-04-0334 |
ISSN: | 1059-1524 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) |
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