Species identification and detection of fungi in biological materials by FTIR microscopy
Fecha
2006Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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nodoi-noplumx
|
Resumen
FTIR spectroscopy provides the opportunity to simultaneously detect many
molecular bonds or functional groups of different polysaccharides, proteins,
lipids, aromatic and other compounds. The measurement principle is based
on the absorption of infrared light by dipolar molecular bonds. In combination
with microscopy, local resolution of the chemical composition is possible.
Each absorption p ...
[++]
FTIR spectroscopy provides the opportunity to simultaneously detect many
molecular bonds or functional groups of different polysaccharides, proteins,
lipids, aromatic and other compounds. The measurement principle is based
on the absorption of infrared light by dipolar molecular bonds. In combination
with microscopy, local resolution of the chemical composition is possible.
Each absorption point or peak in the spectrum can be integrated to create
an image of the distribution of the corresponding compound. We use
FTIR-microscopy in order to detect fungi in plant tissues such as in infected
wood and in mycorhizal roots. For the development of a fast and inexpensive
method for localisation and identification of fungi, differences between
FTIR measurements of fungi and plant cells are characterized. In addition,
FTIR spectra of different fungi are compared. Beech wood blocks were infected
with Trametes versicolor and with Schizophyllum commune and FTIR
spectra in sections of the infected wood determined. Cluster analysis revealed
major differences between FTIR spectra recorded from wood fibres and
empty vessel lumina and spectra from fungal mycelium, irrespectively of
whether grown on the surface of wood or inside vessel lumina. Species specific
clustering of spectra of fungal mycelium grown on the wood surface and
inside vessel lumina demonstrated the potential of FTIR microscopy to
identify fungal mycelium in wood. Currently, we are sampling FTIR spectra
from various basidiomycetes in order to define species according to their specific
FTIR spectra.
The work is supported in frame of the Lower Saxony Competence Network
for Sustainable Timber Utilisation (NHN) by the Ministry of Culture
of Lower Saxony and EFRE. The group of UK is funded by the DBU.
MNG holds a PhD studentship from CONACYT, Mexico. [--]
Materias
FTIR microscopy,
Species identification,
Detection of fungi
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
Entidades Financiadoras
The work is supported in frame of the Lower Saxony Competence Network
for Sustainable Timber Utilisation (NHN) by the Ministry of Culture
of Lower Saxony and EFRE. The group of UK is funded by the DBU.
MNG holds a PhD studentship from CONACYT, Mexico.