Abstract
Seagrasses are monocotyledonous marine flowering plants that are considered lungs of the sea and are the most intense carbon sinks on the planet, delivering a range of ecologically and economically valuable biological services. In this study, we report the chemical fingerprint of Zostera muelleri using an untargeted metabolomic approach. High-performance liquid chromatography-mass spectrometry (HPLC-MS) and gas chromatography-mass spectrometry (GC-MS) were performed to study the metabolic profile of Z. muelleri. A total of 98 metabolites belonging to various chemical classes including flavonoids, phenolics, lipids, fatty acids, sugar alcohols and amino acids were identified, including two characteristic marker compounds of the genus, zosteric acid and rosmarinic acid. Chromatographic profiling yield a comprehensive map for the chemical constituents of Z. muelleri, and this method can be used as an effective and convenient approach to gain insights into the chemical composition of other seagrasses.
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References
Achamlale S, Rezzonico B, Grignon-Dubois M (2009) Rosmarinic acid from beach waste: isolation and HPLC quantification in Zostera detritus from Arcachon lagoon. Food Chem 113(4):878–883
Barros L, Dueñas M, Dias MI, Sousa MJ, Santos-Buelga C, Ferreira ICFR (2013) Phenolic profiles of cultivated, in vitro cultured and commercial samples of Melissa officinalis L. infusions. Food Chem 136(1):1–8
Cuny P, Serve L, Jupin H, Boudouresque C-F (1995) Water soluble phenolic compounds of the marine phanerogam Posidonia oceanica in a Mediterranean area colonised by the introduced chlorophyte Caulerpa taxifolia. Aquat Bot 52(3):237–242
Custódio L, Laukaityte S, Engelen AH, Rodrigues MJ, Pereira H, Vizetto-Duarte C et al (2016) A comparative evaluation of biological activities and bioactive compounds of the seagrasses Zostera marina and Zostera noltei from southern Portugal. Nat Prod Res 30(6):724–728
De Leeuw JW, Irene W, Rijpstra C, Nienhuis PH (1995) Free and bound fatty acids and hydroxy fatty acids in the living and decomposing eelgrass Zostera marina L. Org Geochem 23(8):721–728
Duarte CM, Chiscano CL (1999) Seagrass biomass and production: a reassessment. Aquat Bot 65(1–4):159–174
Enerstvedt KH, Jordheim M, Andersen M (2016) Isolation and identification of flavonoids found in Zostera marina collected in Norwegian coastal waters. Am J Plant Sci 7(7):1163–1172
Engle JM, Miller KA (2003) Distribution and morphology of eelgrass (Zoster marina L.) at the California Channel Islands. In: Garcelon DK, Schwemm CA (eds) Sixth California Island symposium. Institute for Wildlife Studies, Arcata, pp 1584–1590
Farag MA, Porzel A, Wessjohann LA (2012) Comparative metabolite profiling and fingerprinting of medicinal licorice roots using a multiplex approach of GC–MS, LC–MS and 1D NMR techniques. Phytochemistry 76:60–72
Gillan FT, Hogg RW, Drew EA (1984) The sterol and fatty acid compositions of seven tropical seagrasses from North Queensland, Australia. Phytochemistry 23(12):2817–2821
Grignon-Dubois M, Rezzonico B (2012) First phytochemical evidence of chemotypes for the seagrass Zostera noltii. Plants 1(1):27–38
Gu J, Weber K, Klemp E, Winters G, Franssen SU, Wienpahl I et al (2012) Identifying core features of adaptive metabolic mechanisms for chronic heat stress attenuation contributing to systems robustness. Integr Biol 4(5):480–493
Hasler-Sheetal H, Castorani MCN, Glud RN, Canfield DE, Holmer M (2016) Metabolomics reveals cryptic interactive effects of species interactions and environmental stress on nitrogen and sulfur metabolism in seagrass. Environ Sci Technol 50(21):11602–11609
Hasler-Sheetal H, Fragner L, Holmer M, Weckwerth W (2015) Diurnal effects of anoxia on the metabolome of the seagrass Zostera marina. Metabolomics 11(5):1208–1218
Haznedaroglu MZ, Zeybek U (2007) HPLC determination of chicoric acid in leaves of Posidonia oceanica. Pharm Biol 45(10):745–748
Kawasaki W, Matsui K, Akakabe Y, Itai N, Kajiwara T (1998) Volatiles from Zostera marina. Phytochemistry 47(1):27–29
Kim JH, Cho YH, Park SM, Lee KE, Lee JJ, Lee BC et al (2004) Antioxidants and inhibitor of matrix metalloproteinase-1 expression from leaves of Zostera marina L. Arch Pharm Res 27(2):177–183
Kumar M, Kuzhiumparambil U, Pernice M, Jiang Z, Ralph PJ (2016) Metabolomics: an emerging frontier of systems biology in marine macrophytes. Algal Res 16:76–92
Laabir M, Grignon-Dubois M, Masseret E, Rezzonico B, Soteras G, Rouquette M et al (2013) Algicidal effects of Zostera marina L. and Zostera noltii Hornem. Extracts on the neuro-toxic bloom-forming dinoflagellate Alexandrium catenella. Aquat Bot 111:16–25
Orth RJ, Carruthers TJB, Dennison WC, Duarte CM, Fourqurean JW, Heck KL et al (1976) A global crisis for seagrass ecosystems. Bioscience 56(12):987–996
Papenbrock J (2012) Highlights in seagrasses phylogeny, physiology, and metabolism: what makes them special? ISRN Botany 2012:15.17
Quackenbush RC, Bunn D, Lingren W (1986) HPLC determination of phenolic acids in the water-soluble extract of Zostera marina L. (eelgrass). Aquat Bot 24(1):83–89
Ribeiro PR, Fernandez LG, de Castro RD, Ligterink W, Hilhorst HW (2014) Physiological and biochemical responses of Ricinus communis seedlings to different temperatures: a metabolomics approach. BMC Plant Biol 14(1):223
Subhashini P, Dilipan E, Thangaradjou T, Papenbrock J (2013) Bioactive natural products from marine angiosperms: abundance and functions. Nat Prod and Bioprospect 3(4):129–136
Taamalli A, Arráez-Román D, Abaza L, Iswaldi I, Fernández-Gutiérrez A, Zarrouk M et al (2015) LC-MS-based metabolite profiling of methanolic extracts from the medicinal and aromatic species Mentha pulegium and Origanum majorana. Phytochem Anal 26(5):320–330
Todd JS, Zimmerman RC, Crews P, Alberte RS (1993) The antifouling activity of natural and synthetic phenol acid sulphate esters. Phytochemistry 34(2):401–404
Vaclavik L, Lacina O, Hajslova J, Zweigenbaum J (2011) The use of high performance liquid chromatography–quadrupole time-of-flight mass spectrometry coupled to advanced data mining and chemometric tools for discrimination and classification of red wines according to their variety. Anal Chim Acta 685(1):45–51
Vergeer LHT, Develi A (1997) Phenolic acids in healthy and infected leaves of Zostera marina and their growth-limiting properties towards Labyrinthula zosterae. Aquat Bot 58(1):65–72
Wang J, Pan X, Han Y, Guo D, Guo Q, Li R (2012) Rosmarinic acid from eelgrass shows nematicidal and antibacterial activities against pine wood nematode and its carrying bacteria. Mar Drugs 10(12):2729–2740
Zidorn C (2016) Secondary metabolites of seagrasses (Alismatales and Potamogetonales; Alismatidae): chemical diversity, bioactivity, and ecological function. Phytochemistry 124:5–28
Acknowledgement
LC-MS analysis and tentative identification of molecules using MA database was carried out by UK and Dr. Adrian Lutz, Metabolomics Australia, School of BioSciences, University of Melbourne. Tentative annotations using Metlin database and interpretation of mass spectra was carried out by UK. MK collected the seagrass samples and processed them for GC and LC-MS analysis. GC-MS profiling was carried out by Dorothee R Hahne, UWA Centre for Metabolomics, The University of Western Australia. MK is grateful to the Australian Research Council for awarding him Discovery Early Career Research Award (DECRA Fellowship, DE150100461-2015).
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Kuzhiumparambil, U., Kumar, M., Ralph, P. (2017). Gas and Liquid Chromatography-Mass Spectrometry-Based Metabolic Profiling of Marine Angiosperm Zostera muelleri (Alismatales, Zosteraceae). In: Kumar, M., Ralph, P. (eds) Systems Biology of Marine Ecosystems. Springer, Cham. https://doi.org/10.1007/978-3-319-62094-7_9
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DOI: https://doi.org/10.1007/978-3-319-62094-7_9
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