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Poly-ß-hydroxybutyrate content and dose of the bacterial carrier for Artemia enrichment determine the performance of giant freshwater prawn larvae

  • Environmental biotechnology
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Abstract

The beneficial effects of poly-β-hydroxybutyrate (PHB) for aquaculture animals have been shown in several studies. The strategy of applying PHB contained in a bacterial carrier has, however, hardly been considered. The effect of administering PHB-accumulated Alcaligenes eutrophus H16 containing 10 or 80 % PHB on dry weight, named A10 and A80, respectively, through the live feed Artemia was investigated on the culture performance of larvae of the giant freshwater prawn (Macrobrachium rosenbergii). Feeding larvae with Artemia nauplii enriched in a medium containing 100 and 1,000 mg L−1 A80 significantly increased the survival with about 15 % and the development of the larvae with a larval stage index of about 1 as compared to feeding non-enriched Artemia. The survival of the larvae also significantly increased with about 35 % in case of a challenge with Vibrio harveyi. The efficiency of these treatments was equal to a control treatment of Artemia enriched in an 800 mg L−1 PHB powder suspension, while Artemia enriched in 10 mg L−1 A80, 100 mg L−1 A10, and 1,000 mg L−1 A10 did not bring similar effects. From our results, it can be concluded that PHB supplemented in a bacterial carrier (i.e., amorphous PHB) can increase the larviculture efficiency of giant freshwater prawn similar to supplementation of PHB in powdered form (i.e., crystalline PHB). When the level of PHB in the bacterial carrier is high, similar beneficial effects can be achieved as crystalline PHB, but at a lower live food enrichment concentration expressed on PHB basis.

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Acknowledgments

The financial support by the Vietnamese government project “Main program on development and application of Biological technology in agriculture and development of rural country to the year 2020” and the Ghent University project “Host-microbial interactions in aquatic production (BOF12/GOA/022)” is highly appreciated. Peter DS is supported as a post-doctoral researcher by the Research Foundation - Flanders (FWO) (Belgium).

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Authors and Affiliations

  1. Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Rozier 44, 9000, Ghent, Belgium

    Truong Quoc Thai, Mathieu Wille, Patrick Sorgeloos, Peter Bossier & Peter De Schryver

  2. Research Institute for Aquaculture, No. 3, 33 Dang Tat, Nha Trang City, Vietnam

    Truong Quoc Thai

  3. VITO, Boeretang 200, 2400, Mol, Belgium

    Linsey Garcia-Gonzalez

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  1. Truong Quoc Thai
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  2. Mathieu Wille
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  3. Linsey Garcia-Gonzalez
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  4. Patrick Sorgeloos
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  6. Peter De Schryver
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Correspondence to Peter De Schryver.

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Thai, T.Q., Wille, M., Garcia-Gonzalez, L. et al. Poly-ß-hydroxybutyrate content and dose of the bacterial carrier for Artemia enrichment determine the performance of giant freshwater prawn larvae. Appl Microbiol Biotechnol 98, 5205–5215 (2014). https://doi.org/10.1007/s00253-014-5536-7

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