Abstract
In August 2008, a massive epizootic occurred among Portunus trituberculatus reared together with Palaemon carincauda Holthuis and Sinonovacula constricta in the seawater pond of Ningang farm, Rudong district, Jiangsu province, China. The disease occurred in crabs from juveniles to adults, and the mortality rate reached 30–40%. The diseased crabs exhibited lethargy, hepatopancreas turgidity, and elevated levels of turbid hemolymph. A gram-negative, rod-shaped bacterium (designated as strain LGS-1) was isolated from the ascitic fluid of the diseased and moribund crabs and was confirmed as the causative agent by our infectivity study. We conducted morphological and biochemical characterization of LGS-1, and sequenced the 16S rRNA gene, which led us to identify the bacterium as Vibrio metschnikovii. Drug sensitivity tests showed that this pathogenic bacterium is sensitive to florfenicol, orfloxacin, and SXT, but completely resistant to antibacterial drugs like gentamicin, erythrocin, and acheomycin. This is the first report on Vibrio metschnikovii as a virulent pathogen for Portunus trituberculatus.
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References
Amaro C, Biosca EG, Esteve C, Fouz B, Toranzo AE (1992) Comparative study of phenotypic and virulence properties in Vibrio vulnificus biotype 1 and 2 obtained from a European eel farm experiencing mortalities. Dis Aquat Org 13:29–35
Chakraborty S, Nair GB, Shinoda S (1997) Pathogenic vibrios in the natural aquatic environment. Rev Environ Health 12:63–80
Dong XZ, Cai MY (2001) Manual of familiar bacterium identification. Science Press, Beijing (in Chinese)
Elhadi N, Radu S, Chen CH, Nishibuchi M (2004) Prevalence of potentially pathogenic Vibrio species in the seafood marketed in Malaysia. J Food Prot 67:1469–1475
He CW, Song XQ, Tang HY (2003) Isolation and identification of the pathogen from Mauremys mutica. Chinese J Vet Sci Tech 33:55–56
Hervio-Heath D, Colwell RR, Derrien A, Robert-Pillot A, Fournier JM, Pommerpuy M (2002) Occurrence of pathogenic Vibrio in coastal areas of France. J Appl Microbiol 92:1122–1135
Jean JW, Rajashekaraiah KR, Farmer JJ, Hickman FW, Morris JG, Kallick CA (1981) Vibrio metschnikovii bacteremia in a patient with cholecystitis. J Clin Microbiol 14:711–712
Jensen S, Bergh Ø, Enger Ø, Hjeltnes B (2002) Use of PCR-RFLP for genotyping 16S rRNA and characterizing bacteria cultured from halibut fry. Can J Microbiol 48:379–386
Jin S, Li Z, Chen YE (2003) A new disease in Portunustrituberculatus culture. J Aquacu 24:24 (in Chinese)
Khemiri F, Durkovsky J (1983) Characteristics of Vibrio metschnikovii isolated in Tunisia. Arch Inst Pasteur Tunis 60:13–19
Kiyokunmi U, Koujis-Uzukai ND, Katswai S (1994) Vibriosis of swimming crab Portunus trituberculatus in larviculture. J World Aquac Soc 25:50–54
Liu Q, Li HY, Wang Q, Liu P, Dai FY, Li J (2007) Identification and phylogenetic analysis of a strain of Vibrio alginolyticus, a pathogen in Portunus trituberculatus with toothpaste disease. Mar Fish Res 28:9–13
Miyake M, Honda T, Miwatani T (1988) Purification and characterization of Vibrio metschnikovii cytolysin. Infect Immun 56:954–960
Mo ZL, Mao YX, Chen SY, Zhang ZD, Xu YL, Zhang PJ (2003) Identification and phylogenetic analysis of one pathogenic bacterium associated with swollen abdomen of cultured flounder (Paralichthys olivaceus) larvae. Oceanol Limnol Sin 34:131–141 (in Chinese)
Munro PM, Brahic G, Clément RL (1994) Seawater effects on various Vibrio species. Microbios 77:191–198
Ni CZ, Ye DZ, Lin YS (1995) Halophilic vibrios in the water of Xiamen Harbor and Daya Bay. Acta Oceanol Sin 14:413–420
Scoglio ME, Di PA, Picerno I, Delia S, Mauro A, Lagana P (2001) Virulence factors in Vibrios and Aeromonads isolated from seafood. New Microbiol 24:273–280
Shi YS, Tong GZ, Gu SG (2004) Isolation and identification of the Photobacterium damselae subsp. piscicida from Portunus trituberculatus. Chinese J Health Lab Tech 14:476
Shi H, Xu WJ, Xu HX, Shi HD (2008) The 18S rRNA sequencing and analysis of a pathogenic yeast, a causative agent of emulsification disease in Portunus trituberculatus. Mar Fish Res 29:34–38
Takashi A, Tadatoshi K, Satoshi W (1983) Drug resistance and R plamids in Vibrio anguillarum isolated in cultured Ayu (Plecoglossus altivelis). J Fish Dis 28:1–9
Urdaci MC, Marchand M, Grimont PA (1988) Species of the genus Vibrio associated with marine products from Arachon Bay. Ann Inst Pasteur Microbiol 139:351–362
Wang GL, Zheng TL, Lu TX (2002) Community diversity of bacteria in digestive tract of mud snail and its rearing shoal. Acta Oceanol Sin 21:529–534
Wang Gl, JIN S, Chen Y, Li Z (2006) Study on pathogens and pathogenesis of emulsification disease of Portunus intritubercularus. Adv Mar Sci 24:526–561
Wang GX, Huang ZR, Yuan M (2007) Isolation and identification of the pathogen of milk disease cultured in swimming crab Portunus intritubercularus (portunidae). J Northw A F Univ 35:29–33
WJ XU, SHI H, HX XU, Hamish S (2007) Preliminary study on the Hematodimium infection in cultured Portunus trituberculatus. Acta Hydrobio Sin 31:637–642
Wu YL, Liu SZ (1995) Studies on the causes of death of Portunus trituberculatus (Miers). J Oceano East Chi Sea 13:37–40
Xu HS, Li J, Phyllis B (1998) Occurrence and distribution of vibrios in fishes and shellfishes in coastal waters of HongKong. Acta Oceanol Sin 17:545–553
Xu WJ, Xu HX, Jin W (2003) Study on pathogen of emulsification disease of Portunus trituberculatus. J Zhejiang Coll Oceano 22:209–213
Yalcinkaya F, Ergin C, Agalar C, Kaya S, Aksoylar MY (2003) The presence and antimicrobial susceptibilities of human-pathogen Vibrio spp. Isolated from blue crub in Belek tourism coast. Turkey J Environ Health Res 3:95–98
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We thank Yan Jin for her skillful assistance.
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Wan, X., Shen, H., Wang, L. et al. Isolation and characterization of Vibrio metschnikovii causing infection in farmed Portunus trituberculatus in China. Aquacult Int 19, 351–359 (2011). https://doi.org/10.1007/s10499-011-9422-3
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DOI: https://doi.org/10.1007/s10499-011-9422-3