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Toxicity testing of cosmetic ingredients using gametophyte beads of the brown alga Undaria pinnatifida (Laminariales, Phaeophyta)

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Abstract

A 6-h toxicity test of cosmetic ingredients (methylparaben, 2-phenoxyethanol, sodium dodecyl sulfate, triethanolamine) was developed, based on the photosynthetic maximum quantum yield (Fv/Fm) of immobilized gametophytes of the brown macroalga Undaria pinnatifida. From calculated EC50 values, the toxicity ranking of the tested ingredients is: SDS (0.0060%) > MP (0.0634%) > 2-PE (0.2418%) > TEA (3.7023%). Compared to the results from conventional endpoints with other ecotoxicity test organisms, measurements of Fv/Fm is a more sensitive indicator of the toxic effects of cosmetic ingredients. The present technique is simple, rapid, practical, accurate, and requires little space to carry out. This novel method will be a useful tool for assessing the toxicity of a wide range of cosmetic ingredients once the respective sensitivities are fully established.

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Funding

This work was supported by a Grant of Incheon National University Research (grant no. 2012-0341).

Author information

Authors and Affiliations

  1. Department of Marine Science, Incheon National University, Academy-ro 119, Yeonsu-gu, Incheon, 22012, Republic of Korea

    Hojun Lee & Taejun Han

  2. Department of Cosmetic Science and Management, Incheon National University, Academy-ro 119, Yeonsu-gu, Incheon, 22012, Republic of Korea

    Juseon Lee

  3. School of Marine Science & Engineering, Plymouth University, Plymouth, Devon, PL4 8AA, UK

    Murray T. Brown

  4. Ghent University Global Campus, Songdomunhwa-ro 119, Yeonsu-gu, Incheon, 21985, Republic of Korea

    Jihae Park & Taejun Han

  5. Phycology Research Group and Center for Molecular Phylogenetics and Evolution, Ghent University, Krijgslaan 281 (S8), 9000, Ghent, Belgium

    Christophe Vieira

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  2. Juseon Lee
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  3. Murray T. Brown
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  4. Jihae Park
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  5. Christophe Vieira
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  6. Taejun Han
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Correspondence to Taejun Han.

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Lee, H., Lee, J., Brown, M.T. et al. Toxicity testing of cosmetic ingredients using gametophyte beads of the brown alga Undaria pinnatifida (Laminariales, Phaeophyta). J Appl Phycol 31, 2011–2023 (2019). https://doi.org/10.1007/s10811-018-1669-x

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  • DOI: https://doi.org/10.1007/s10811-018-1669-x

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