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Plant growth-promoting rhizobacteria enhance the growth and Cd uptake of Sedum plumbizincicola in a Cd-contaminated soil

  • Soils, Sec 5 • Soil and Landscape Ecology • Research Article
  • Published:
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Abstract

Purpose

This study aimed to isolate plant growth-promoting rhizobacteria (PGPR) that exhibit heavy metal resistance to examine their influence on Cd uptake and soil microbial community structure during phytoremediation.

Materials and methods

Heavy metal-tolerant PGPR were isolated from the roots of possible hyperaccumulators using plates with 1-aminocyclopropane-1-carboxylate (ACC) as sole nitrogen source. Minimal inhibitory concentrations (MICs) of each isolate were determined by the plate dilution method. The impacts of isolated PGPR on the growth and Cd accumulation of Sedium plumbizincicola were conducted in a pot experiment. In addition, the effect of PGPR inoculation on the microbial community during phytoextraction by S. plumbizincicola was studied by 454 pyrosequencing.

Results and discussion

A total of nine Cd-resistant strains were isolated from the roots of Cd accumulators, and their plant growth-promoting activities were characterized. Isolates were able to produce indole-3-acetic acid (IAA) (28–133 mg L−1) and solubilize phosphate (65–148 mg L−1). In a pot experiment, the inoculation of isolates NSX2 and LCR1 significantly enhanced the growth of and uptake of Cd by the Cd hyperaccumulator S. plumbizincicola. 454 pyrosequencing revealed that the inoculation of the PGPR lead to a decrease in microbial community diversity in the rhizopshere during phytoextraction. Specifically, indigenous heavy metal-tolerant PGPR such as Actinospica, Bradyrhizobium, Rhizobium, Mesorhizobium, and Mycobacterium were selectively enriched in the treatments in which PGPR were added. It is suggested that a unique constitution of microbial communities in inoculated treatments plays a key role in enhancing Cd phytoremediation.

Conclusion

Inoculation of strains Rhodococcus erythropolis NSX2 and Cedecea davisae LCR1 could promote S. plumbizincicola growth and enhance the remediation efficiency. The introduced PGPR could also affect the indigenous microbial community structure and the diversity in Cd-contaminated soil during phytoremediation.

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Acknowledgments

We thank the National High Technology Research and Development Program of China (863 Program) (No. 2012AA06A204), Jiangsu Provincial Natural Science Foundation of China (BK2012891), the International Scientific Collaborative Program of the Ministry of Science and Technology of China (2010DFA92360), and National Natural Science Foundation of China (41001182) for financial support. Tang and Liu were also supported by an Australian Research Council Linkage Project (LP100100800).

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Authors and Affiliations

  1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China

    Wuxing Liu, Qingling Wang, Beibei Wang, Jinyu Hou & Yongming Luo

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Beibei Wang & Jinyu Hou

  3. Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China

    Yongming Luo

  4. Centre for AgriBioscience, La Trobe University, Melbourne Campus, Bundoora, Victoria, 3086, Australia

    Caixian Tang

  5. Department of Microbiology, La Trobe University, Melbourne Campus, Bundoora, Victoria, 3086, Australia

    Ashley E. Franks

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  1. Wuxing Liu
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  2. Qingling Wang
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Correspondence to Wuxing Liu.

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Responsible editor: Jizheng He

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Liu, W., Wang, Q., Wang, B. et al. Plant growth-promoting rhizobacteria enhance the growth and Cd uptake of Sedum plumbizincicola in a Cd-contaminated soil. J Soils Sediments 15, 1191–1199 (2015). https://doi.org/10.1007/s11368-015-1067-9

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  • DOI: https://doi.org/10.1007/s11368-015-1067-9

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