Planta Med 2019; 85(18): 1469
DOI: 10.1055/s-0039-3399848
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

A triterpene glycoside from the black sea cucumber Holothuria atra

YE Puspitasari
1   Natural Product & Food Research and Analysis (Natura), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp,, Universiteitsplein 1, 2610 Antwerp, Belgium
2   Fisheries and Marine Sciences Faculty, University of Brawijaya,, Malang 65145 Indonesia
,
E Tuenter
1   Natural Product & Food Research and Analysis (Natura), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp,, Universiteitsplein 1, 2610 Antwerp, Belgium
,
K Foubert
1   Natural Product & Food Research and Analysis (Natura), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp,, Universiteitsplein 1, 2610 Antwerp, Belgium
,
L Pieters
1   Natural Product & Food Research and Analysis (Natura), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp,, Universiteitsplein 1, 2610 Antwerp, Belgium
,
T De Bruyne
1   Natural Product & Food Research and Analysis (Natura), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp,, Universiteitsplein 1, 2610 Antwerp, Belgium
,
N Hermans
1   Natural Product & Food Research and Analysis (Natura), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp,, Universiteitsplein 1, 2610 Antwerp, Belgium
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

Sea cucumber is a soft-bodied, worm-like, echinoderm. It is consumed in its dried form, which is called “bêche-de-mer”. Many reports described the presence of triterpene glycosides (saponins) in sea cucumbers, and especially the Cuvierian tubules are a rich source of this type of compounds [1],[2]. The black sea cucumber, Holothuria atra Jaeger, is one of the commercially important species in Indonesia. However, in contrast to most species of sea cucumber, H. atra does not possess Cuvierian tubules, but saponins were previously isolated from its body wall [3]. In this project, an extensive study of the composition of the body wall of H. atra is carried out, with special interest in its saponin content. Black sea cucumbers were collected in East Java, Indonesia, dried, and extracted with dichloromethane and 80% methanol. Fractionation was carried out by means of liquid partitioning and column chromatography (diaion HP20). Finally, semi-preparative HPLC-DAD-MS allowed for the purification of various saponins, according to their 1H-NMR spectra. Up till now, the structure of one compound could be fully elucidated, based on 1D and 2D NMR and HRMS data. All NMR data were in correspondence to the data reported by Kitagawa, for desulfated holothurin B [4]. Moreover, the HRMS data of this compound were recorded for the first time and confirmed the identification of the compound as desulfated holothurin B (m/z 803.4250, [M+Na]+; calculated for C41H64O14Na: 803.4194; and m/z 485.3267, aglycon). Further research with regard to the structure elucidation of other purified compounds is ongoing.

Zoom Image
Fig. 1 Desulfated holothurin B of black sea cucumber Holothuria atra
 

  • References

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