Serum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study
Permanent lenke
https://hdl.handle.net/10037/24002Dato
2021-08-02Type
Journal articleTidsskriftartikkel
Peer reviewed
Forfatter
Myklebust, Mette Pernille; Thor, Anna; Rosenlund, Nina Benedikte; Gjengstø, Peder; Karlsdottir, Åsa; Brydøy, Marianne; Bercea, Bogdan Stefan; Olsen, Christian; Johnson, Ida; Berg, Mathilde I.; Langberg, Carl; Andreassen, Kristine; Kjellman, Anders; Haugnes, Hege Sagstuen; Dahl, OlavSammendrag
MicroRNA-371a-3p (miR371) has been suggested as a sensitive biomarker in testicular germ cell
cancer (TGCC). We aimed to compare miR371 with the classical biomarkers α-fetoprotein (AFP) and
β-human chorionic gonadotropin (hCGβ). Overall, 180 patients were prospectively enrolled in the
study, with serum samples collected before and after orchiectomy. We compared the use of digital
droplet PCR (RT-ddPCR) with the quantitative PCR used by others for detection of miR371. The novel
RT-ddPCR protocol showed high performance in detection of miR371 in serum samples. In the study
cohort, miR371 was measured using RT-ddPCR. MiR371 detected CS1 of the seminoma and the nonseminoma sub-types with a sensitivity of 87% and 89%, respectively. The total sensitivity was 89%.
After orchiectomy, miR371 levels declined in 154 of 159 TGCC cases. The ratio of miR371 pre- and
post-orchiectomy was 20.5 in CS1 compared to 6.5 in systemic disease. AFP and hCGβ had sensitivities
of 52% and 51% in the non-seminomas. MiR371 is a sensitive marker that performs better than the
classical markers in all sub-types and clinical stages. Especially for the seminomas CS1, the high
sensitivity of miR371 in detecting TGCC cells may have clinical implications.
Forlag
NatureSitering
Myklebust, Thor, Rosenlund, Gjengstø, Karlsdottir, Brydøy, Bercea, Olsen, Johnson, Berg, Langberg, Andreassen, Kjellman, Haugnes h, Dahl. Serum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study. Scientific Reports. 2021Metadata
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