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Influence of Different Additives and Their Concentrations on Corneal Toxicity and Antimicrobial Effect of Benzalkonium Chloride
http://hdl.handle.net/10069/35452
http://hdl.handle.net/10069/354523f48ac3f-c44d-4278-9ec7-856aa06851ed
名前 / ファイル | ライセンス | アクション |
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ISYK681_Onizuka.pdf (1.1 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2015-06-05 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Influence of Different Additives and Their Concentrations on Corneal Toxicity and Antimicrobial Effect of Benzalkonium Chloride | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | benzalkonium chloride | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | polyoxyethylene hydrogenated castor oil 40 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | polysorbate 80 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cornea | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | antimicrobial | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | transepithelial electrical resistance | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
鬼塚, 尚子
× 鬼塚, 尚子 |
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著者別名 | ||||||
姓名 | Onizuka, Naoko | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Purpose: The aim of this study was to examine the ophthalmic additives responsible for modulating acute corneal epithelial toxicity induced by benzalkonium chloride (BAC) and investigate the ability of polyoxyethylene hydrogenated castor oil 40 (HCO-40) and polysorbate 80 (PS-80) to reduce the corneal toxicity and antimicrobial effects of BAC. Methods: Cytotoxicity of the additives, which included glycerin, polyvinyl alcohol, propylene glycol, polyethylene glycol, and PS-80, on rabbit corneal epithelial cells was examined using the cell proliferation assay in the presence and absence of 0.02% BAC. The corneal transepithelial electrical resistance change after a 60-second exposure to HCO-40 or PS-80 mixed with 0.02% BAC was measured in living rabbits. Corneal damage was examined using scanning electron microscopy. The antimicrobial activities of HCO-40 and PS-80 with 0.02% BAC against Staphylococcus aureus, Propionibacterium acnes, Pseudomonas aeruginosa, Escherichia coli, and Streptococcus pneumoniae were assessed. Results: Of all the tested additives, only PS-80 could prevent the BAC-induced cytotoxicity. Corneal epithelial barrier function disorder caused by 0.02% BAC was significantly alleviated by either PS-80 or HCO-40 in a concentration-dependent manner. Scanning electron microscopy images showed an improvement of BAC-induced corneal epithelial toxicity after the addition of HCO-40 or PS-80. The antimicrobial effect of the BAC against P. aeruginosa, E. coli, and S. pneumoniae was reduced after adding HCO-40 or PS-80. Conclusions: HCO-40 and PS-80 reduce acute corneal toxicity and the antimicrobial effect of BAC. Possible interactions between BAC and other additives should be taken into consideration when evaluating the toxicity and antibacterial properties of BAC. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第681号 学位授与年月日:平成26年3月20日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Onizuka Naoko, Uematsu Masafumi, Kusano Mao, Sasaki Hitoshi, Suzuma Kiyoshi, Kitaoka Takashi | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Cornea, 33(5), pp.521-526; 2014 | |||||
書誌情報 |
Cornea 巻 33, 号 5, p. 521-526, 発行日 2014-03-20 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 02773740 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1097/ICO.0000000000000086 | |||||
権利 | ||||||
権利情報 | c 2014 by Lippincott Williams & Wilkins. | |||||
権利 | ||||||
権利情報 | This is a non-final version of an article published in final form in Cornea, 33(5), pp.521-526; 2014. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | 塩化ベンザルコニウムに起因する角膜上皮障害と防腐効果に対する異なる2種類の添加剤とその濃度の影響 | |||||
出版者 | ||||||
出版者 | Lippincott Williams & Wilkins. | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/34630 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2014-03-20 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第681号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2014-03-20) |