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Rat Hepatocyte Spheroids Formed on Temperature-Responsive PIPAAm Polymer-Grafted Surface Maintain Long-Term Differentiated Hepatocyte Function
http://hdl.handle.net/10069/35775
http://hdl.handle.net/10069/357750cd818ac-9131-4f22-b32a-e1d5315b12ef
名前 / ファイル | ライセンス | アクション |
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ISYK705_Tomonaga.pdf (1.0 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2015-08-18 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Rat Hepatocyte Spheroids Formed on Temperature-Responsive PIPAAm Polymer-Grafted Surface Maintain Long-Term Differentiated Hepatocyte Function | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hepatocyte | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Temperature-responsive Surface | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Spheroid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PIPAAm | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
朝長, 哲生
× 朝長, 哲生 |
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著者別名 | ||||||
姓名 | Tomonaga, Tetsuo | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background. Although the development of a practicable culture method to maintain long-term hepatocyte function is highly desirable, hepatocytes lose their liver-specific functions rapidly on regular collagen-coated dishes. In this study, we evaluated the efficacy of a special cell culture dish coated with the temperature-responsive polymer poly(N-isopropylacrylamide) (PIPAAm), and observed the morphological and functional changes of hepatocytes on changing the amount of polymer. Methods. Culture plates with varying amounts of PIPAAm polymer (1.5~3.5 times that in the commercially available temperatureresponsive polymer-containing dish (UpCellR, CellSeed, Tokyo, Japan)) were prepared. All dishes were enhanced by adding a layer of rat tail collagen I at a dose of 600 μg/dish for 3 hours. Hepatocytes isolated from Sprague-Dawley rats were cultured in these static culture systems. Morphologic changes and liver-specific functions were evaluated. Results. On day 7 of culture, spheroid formation of hepatocytes was observed in the high-polymer group, with the presence of glycogen and albumin in the spheroid. In the high-polymer group, the rate of albumin production was significantly higher than in the low-polymer group until day 14 of culture (P<0.001). Conclusion. The spheroid formation of hepatocytes cultured in the presence of a high level of PIPAAm showed the long-term maintenance of liver-specific functions in vitro. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第705号 学位授与年月日:平成26年9月3日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Tomonaga Tetsuo, Soyama Akihiko, Yamanouchi Kosho, Takatsuki Mitsuhisa, Kuroki Tamotsu, Koji Takehiko, Eguchi Susumu | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Acta medica Nagasakiensia, 59(1), pp.1-6; 2014 | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 59, 号 1, p. 1-6, 発行日 2014-09-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
権利 | ||||||
権利情報 | c 2014 Nagasaki University School of Medicine | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | 温度応答性ポリマー高グラフト化表面によるラット培養肝細胞の長期機能維持(スフェロイド形成) | |||||
出版者 | ||||||
出版者 | 長崎大学医学部 | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/34877 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2014-09-03 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第705号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2014-09-03) |