WEKO3
アイテム
{"_buckets": {"deposit": "91f413f8-6804-4cb3-bf33-26ed1c51de0b"}, "_deposit": {"created_by": 6, "id": "4985", "owners": [6], "pid": {"revision_id": 0, "type": "depid", "value": "4985"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00004985", "sets": ["24"]}, "author_link": ["115176", "115175"], "item_5_alternative_title_19": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "ヒト乳突蜂巣におけるアポトーシスと層板構造の観察"}]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-02-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageEnd": "1542", "bibliographicPageStart": "1534", "bibliographicVolumeNumber": "34", "bibliographic_titles": [{"bibliographic_title": "Otology \u0026 Neurotology"}]}]}, "item_5_date_granted_64": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2015-02-04"}]}, "item_5_degree_grantor_62": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "Nagasaki University (長崎大学)"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "17301", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_5_degree_name_61": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(医学)"}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Hypothesis: To investigate the localization of apoptotic cells and the lamellar bone structure in mastoid bone tissue, focusing on the mechanism of the development of mastoid pneumatization in humans. Background: The biological mechanism of poor development of the mastoid air cells found in patients with otitis media (OM) has not yet been fully clarified, as there have been few immunohistochemical research studies to examine the cell biology involved. Methods: We evaluated the localization of apoptotic cells and the lamellar bone structure in 112 human mastoid bones harvested during various ear surgeries from 57 patients with OM and 55 patients without OM. We used the TdT-mediated dUTP nick end-labeling (TUNEL) method and a polarizing microscope for observing the apoptotic cells and lamellar bone structure, respectively. Results: The TUNEL-positive cell ratio in an arbitrary 500 cells in the specimen (apoptotic index: AI) was 13.8 in the healthy group and 1.2 in the OM group (Mann-Whitney U test, p \u003c 0.001). From their localization, these apoptotic cells were considered to be osteocytes. The observation of lamellar structures revealed many eroded surfaces in the circumference of the appositional bone in the healthy group. In contrast, apposition of bone without an eroded surface was evident in the bone circumference in the OM group. Conclusion: Apoptosis of osteocytes was significantly suppressed in the mastoid bone of the OM patients. Apoptotic osteocytes may be one of the signals of bone resorption in the process of development of the mastoid air cells. The lamellar structure of the mastoid bone suggested that poor development of the mastoid air cells was caused by decreased resorption of the appositional bone.", "subitem_description_type": "Abstract"}]}, "item_5_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "長崎大学学位論文 学位記番号:博(医歯薬)甲第734号 学位授与年月日:平成27年2月4日", "subitem_description_type": "Other"}, {"subitem_description": "Author: Fujiyama Daisuke, Yamamoto-Fukuda Tomomi, Sibata Yasuaki, Ikeda Tohru, Takahashi Haruo", "subitem_description_type": "Other"}, {"subitem_description": "Citation: Otology \u0026 Neurotology, 34(8), pp.1534-1542; 2013", "subitem_description_type": "Other"}]}, "item_5_description_69": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Nagasaki University (長崎大学), 博士(医学) (2015-02-04)", "subitem_description_type": "Other"}]}, "item_5_dissertation_number_65": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲医歯薬第734号"}]}, "item_5_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "115176", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Fujiyama, Daisuke"}]}]}, "item_5_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Lippincott Williams and Wilkins"}]}, "item_5_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1097/MAO.0b013e3182a435cd", "subitem_relation_type_select": "DOI"}}]}, "item_5_relation_42": {"attribute_name": "関係URI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://hdl.handle.net/10069/35372", "subitem_relation_type_select": "HDL"}}]}, "item_5_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2013 by Otology \u0026 Neurotology, Inc."}, {"subitem_rights": "This is a non-final version of an article published in final form in Otology \u0026 Neurotology, 34(8), pp.1534-1542; 2013"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "15317129", "subitem_source_identifier_type": "ISSN"}]}, "item_5_text_67": {"attribute_name": "学位の種類", "attribute_value_mlt": [{"subitem_text_value": "課程博士"}]}, "item_5_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "藤山, 大祐"}], "nameIdentifiers": [{"nameIdentifier": "115175", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2021-02-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ISYK734_Fujiyama.pdf", "filesize": [{"value": "2.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 2600000.0, "url": {"label": "ISYK734_Fujiyama.pdf", "objectType": "fulltext", "url": "https://nagasaki-u.repo.nii.ac.jp/record/4985/files/ISYK734_Fujiyama.pdf"}, "version_id": "570e8e5e-c9b9-4dc6-b1bc-c1ed96feb4a1"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Apoptosis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Lamellar structure", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Mastoid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Osteocyte", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Otitis media", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Observation of Apoptosis and Bone Lamella Structures in the Human Mastoid", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Observation of Apoptosis and Bone Lamella Structures in the Human Mastoid", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "6", "path": ["24"], "permalink_uri": "http://hdl.handle.net/10069/36124", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2016-01-21"}, "publish_date": "2016-01-21", "publish_status": "0", "recid": "4985", "relation": {}, "relation_version_is_last": true, "title": ["Observation of Apoptosis and Bone Lamella Structures in the Human Mastoid"], "weko_shared_id": -1}
Observation of Apoptosis and Bone Lamella Structures in the Human Mastoid
http://hdl.handle.net/10069/36124
http://hdl.handle.net/10069/3612434fe15d7-aef5-4c4c-9840-99025b654da5
名前 / ファイル | ライセンス | アクション |
---|---|---|
ISYK734_Fujiyama.pdf (2.6 MB)
|
|
Item type | 学位論文 / Thesis or Dissertation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2016-01-21 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Observation of Apoptosis and Bone Lamella Structures in the Human Mastoid | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Apoptosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lamellar structure | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mastoid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Osteocyte | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Otitis media | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
藤山, 大祐
× 藤山, 大祐 |
|||||
著者別名 | ||||||
姓名 | Fujiyama, Daisuke | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Hypothesis: To investigate the localization of apoptotic cells and the lamellar bone structure in mastoid bone tissue, focusing on the mechanism of the development of mastoid pneumatization in humans. Background: The biological mechanism of poor development of the mastoid air cells found in patients with otitis media (OM) has not yet been fully clarified, as there have been few immunohistochemical research studies to examine the cell biology involved. Methods: We evaluated the localization of apoptotic cells and the lamellar bone structure in 112 human mastoid bones harvested during various ear surgeries from 57 patients with OM and 55 patients without OM. We used the TdT-mediated dUTP nick end-labeling (TUNEL) method and a polarizing microscope for observing the apoptotic cells and lamellar bone structure, respectively. Results: The TUNEL-positive cell ratio in an arbitrary 500 cells in the specimen (apoptotic index: AI) was 13.8 in the healthy group and 1.2 in the OM group (Mann-Whitney U test, p < 0.001). From their localization, these apoptotic cells were considered to be osteocytes. The observation of lamellar structures revealed many eroded surfaces in the circumference of the appositional bone in the healthy group. In contrast, apposition of bone without an eroded surface was evident in the bone circumference in the OM group. Conclusion: Apoptosis of osteocytes was significantly suppressed in the mastoid bone of the OM patients. Apoptotic osteocytes may be one of the signals of bone resorption in the process of development of the mastoid air cells. The lamellar structure of the mastoid bone suggested that poor development of the mastoid air cells was caused by decreased resorption of the appositional bone. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第734号 学位授与年月日:平成27年2月4日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Fujiyama Daisuke, Yamamoto-Fukuda Tomomi, Sibata Yasuaki, Ikeda Tohru, Takahashi Haruo | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Otology & Neurotology, 34(8), pp.1534-1542; 2013 | |||||
書誌情報 |
Otology & Neurotology 巻 34, 号 8, p. 1534-1542, 発行日 2015-02-04 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15317129 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1097/MAO.0b013e3182a435cd | |||||
権利 | ||||||
権利情報 | c 2013 by Otology & Neurotology, Inc. | |||||
権利 | ||||||
権利情報 | This is a non-final version of an article published in final form in Otology & Neurotology, 34(8), pp.1534-1542; 2013 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | ヒト乳突蜂巣におけるアポトーシスと層板構造の観察 | |||||
出版者 | ||||||
出版者 | Lippincott Williams and Wilkins | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/35372 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2015-02-04 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第734号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2015-02-04) |