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Identification of Three Novel Fusion Oncogenes, SQSTM1/NTRK3, AFAP1L2/RET, and PPFIBP2/RET, in Thyroid Cancers of Young Patients in Fukushima
http://hdl.handle.net/10069/38111
http://hdl.handle.net/10069/38111ab8617ee-b47d-4bde-8078-61730117bbaa
名前 / ファイル | ライセンス | アクション |
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ISYK981_Iyama.pdf (2.9 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2018-05-01 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Identification of Three Novel Fusion Oncogenes, SQSTM1/NTRK3, AFAP1L2/RET, and PPFIBP2/RET, in Thyroid Cancers of Young Patients in Fukushima | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | fusion gene | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | rearrangement | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | oncogene | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NTRK3 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RET | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | papillary thyroid carcinoma | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
井山, 慶大
× 井山, 慶大 |
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著者別名 | ||||||
姓名 | Iyama, Keita | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: The BRAFV600E mutation is the most frequent genetic abnormality in adult papillary thyroid carcinomas (PTCs). On the other hand, various chromosomal rearrangements are more prevalent in childhood and adolescent PTCs. The aim of the present study was to identify novel rearrangements in PTCs from young patients. Methods: Among 63 postoperative specimens of childhood and adolescent PTCs, which had been discovered by the thyroid ultrasound screening program in Fukushima, nine samples without prevalent known oncogenes, BRAFV600E, RAS, RET/PTC1, RET/PTC3, and ETV6/NTRK3, were analyzed in the current study by quantitative real-time reverse transcription polymerase chain reaction to screen for novel fusion genes by comparing transcript expression between extracellular and kinase domains of ALK, NTRK1, NTRK3, and RET. Results: Of the above nine samples, five samples were suspected to harbor a fusion, and using subsequent 5′ rapid amplification of cDNA end (RACE), two already reported fusion oncogenes, STRN/ALK and TPR/NTRK1, and three novel fusions, SQSTM1/NTRK3, AFAP1L2/RET, and PPFIBP2/RET, were identified. Functional analyses of these three chimeric genes were performed, and their transforming abilities were confirmed through the activation of mitogen-activated protein kinase (MAPK). Conclusions: Three novel fusion oncogenes have been identified in young PTC patients in Fukushima, suggesting that rare fusions may be present among the cases negative for known oncogenes in this age group and that such rearrangements can play a significant role in thyroid carcinogenesis. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第981号 学位授与年月日:平成29年6月7日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Iyama Keita, Matsuse Michiko, Mitsutake Norisato, Rogounovitch Tatiana, Saenko Vladimir, Suzuki Keiji, Ashizawa Mai, Ookouchi Chiyo, Suzuki Satoshi, Mizunuma Hiroshi, Fukushima Toshihiko, Suzuki Shinichi, and Yamashita Shunichi | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Thyroid, 27(6), pp.811-818; 2017 | |||||
書誌情報 |
Thyroid 巻 27, 号 6, p. 811-818, 発行日 2017-06-07 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10507256 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15579077 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1089/thy.2016.0673 | |||||
権利 | ||||||
権利情報 | c 2017 Mary Ann Liebert, Inc. | |||||
権利 | ||||||
権利情報 | Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/thy.2016.0673 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | 福島の若年甲状腺乳頭癌における3つの新規融合癌遺伝子(SQSTM1/NTRK3, AFAP1L2/RET, PPFIBP2/RET)の同定 | |||||
出版者 | ||||||
出版者 | Mary Ann Liebert, Inc. | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/37614 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2017-06-07 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第981号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2017-06-07) |