Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/102856
Author(s): | Berta N. Estevinho Ana M. Damas Pedro Martins Fernando Rocha |
Title: | The influence of microencapsulation with a modified chitosan (water soluble) on ß-galactosidase activity |
Issue Date: | 2014 |
Abstract: | The aim of this work was to investigate the possibility ofproducing microparticles containing b-galactosidase, using a modified chitosan (water soluble) as encapsulating agent. b-galactosidasemicroparticles were prepared by a spray-drying method and werecharacterized in terms of particle size, surface morphology, zetapotential, and stability over a storage period of six months. Microparticles were also analyzed by FTIR spectroscopy and thermogravimetry techniques. Structural analysis of the surface of the particleswas performed by scanning electron microscopy (SEM). SEMresults show that the obtained microparticles have an average diameter smaller than 3.5 lm and a regular shape. The b-galactosidaseactivity decreases when microencapsulated. The parameters Kmand Vmax of the Michaelis-Menten equation were calculated forthe different experimental conditions. The optimal pH ranges from6.4 to 7.2, approximately, depending on the enzyme concentrationin the microcapsule. After six months of storage, the enzyme activitypresents a small decrease, although no significant differences in theappearance, color, and particle size distribution were identified. |
Subject: | Ciências da engenharia e tecnologias Engineering and technology |
Scientific areas: | Ciências da engenharia e tecnologias Engineering and technology |
URI: | https://hdl.handle.net/10216/102856 |
Related Information: | info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/PEst-C/EQB/UI0511/2013/PROJECTO ESTRATÉGICO - UI 511 - 2013-2014/UI0511 |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | FEUP - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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120850.1.pdf | Post-Print version | 1.22 MB | Adobe PDF | View/Open |
120850.pdf Restricted Access | Artigo original publicado | 2.32 MB | Adobe PDF | Request a copy from the Author(s) |
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