Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/103594
Author(s): | Túlio I. S. Oliveira Marcela Oliveira Subramanian Viswanathan M. Fátima Barroso Luísa Barreiros Olga C. Nunes José A. Rodrigues Pedro de Lima Neto Selma E. Mazzetto Simone Morais Cristina Delerue Matos |
Title: | Molinate quantification in environmental water by a glutathione-S-transferase based biosensor |
Issue Date: | 2013 |
Abstract: | A glutathione-S-transferase (GST) based biosensor was developed to quantify the thiocarbamate herbicide molinate in environmental water. The biosensor construction was based on GST immobilization onto a glassy carbon electrode via aminosilane-glutaraldehyde covalent attachment. The principle supporting the use of this biosensor consists of the GST inhibition process promoted by molinate. Differential pulse voltammetry was used to obtain a calibration curve for molinate concentration, ranging from 0.19 to 7.9 mg L-1 and presenting a detection limit of 0.064 mg L-1. The developed biosensor is stable, and reusable during 15 days. The GST-based biosensor was successfully applied to quantify molinate in rice paddy field floodwater samples. The results achieved with the developed biosensor were in accordance with those obtained by high performance liquid chromatography. The proposed device is suitable for screening environmental water analysis and, since no sample preparation is required, it can be used in situ and in real-time measurements. |
Subject: | Química Chemical sciences |
Scientific areas: | Ciências exactas e naturais::Química Natural sciences::Chemical sciences |
URI: | https://hdl.handle.net/10216/103594 |
Related Information: | info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projetos Estratégicos/PEst-C/EQB/UI0511/2013/PROJECTO ESTRATÉGICO - UI 511 - 2013-2014/UI0511 |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | FCUP - Artigo em Revista Científica Internacional FEUP - Artigo em Revista Científica Internacional |
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98115.pdf Restricted Access | Artigo original publicado | 391.73 kB | Adobe PDF | Request a copy from the Author(s) |
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