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https://hdl.handle.net/10216/120374
Author(s): | Albuquerque L. Polónia A.R.M. Barroso C. Froufe H.J.C. Lage O. Lobo-Da-Cunha A. Egas C. Da Costa M.S. |
Title: | Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum bacteroidetes and the description of raineyaceae fam. nov |
Publisher: | Microbiology Society |
Issue Date: | 2018 |
Abstract: | An isolate, designated SPSPC-11T, with an optimum growth temperature of about 50°C and an optimum pH for growth between 7.5 and 8.0, was recovered from a hot spring in central Portugal. Based on phylogenetic analysis of its 16S rRNA sequence, the new organism is most closely related to the species of the genus Thermonema but with a pairwise sequence similarity of <85 %. The isolate was orange-pigmented, formed non-motile long filaments and rod-shaped cells that stain Gram-negative. The organism was strictly aerobic, oxidase-positive and catalase-positive. The major fatty acids were iso- C15:0, iso-C15: 0 2-OH and iso-C17: 0 3-OH. The major polar lipids were one aminophospholipid, two aminolipids and three unidentified lipids. Menaquinone 7 was the major respiratory quinone. The DNA G+C content of strain SPSPC-11T was 37.6 mol% (draft genome sequence). The high quality draft genome sequence corroborated many of the phenotypic characteristics of strain SPSPC-11T. Based on genotypic, phylogenetic, physiological and biochemical characterization we describe a new species of a novel genus represented by strain SPSPC-11T (=CECT 9012T=LMG 29233T) for which we propose the name Raineya orbicola gen. nov., sp. nov. We also describe the family Raineyaceae to accommodate this new genus and species. © 2018 IUMS. |
Subject: | aminophospholipid catalase fatty acid menaquinone oxidoreductase quinone derivative RNA 16S bacterial DNA farnoquinone phospholipid vitamin MK 7 Article bacterial growth bacterium isolate Bacteroidetes DNA base composition gene sequence nonhuman pH phylogeny Portugal priority journal Raineya orbicola RNA sequence scanning electron microscopy temperature thermal spring thermophilic bacterium transmission electron microscopy analogs and derivatives bacterium identification Bacteroidetes chemistry classification DNA sequence genetics isolation and purification microbiology phylogeny pigmentation Bacterial Typing Techniques Bacteroidetes Base Composition DNA, Bacterial Fatty Acids Hot Springs Phospholipids Phylogeny Pigmentation Portugal RNA, Ribosomal, 16S Sequence Analysis, DNA Vitamin K 2 |
URI: | https://hdl.handle.net/10216/120374 |
Source: | International Journal of Systematic and Evolutionary Microbiology, vol. 68(4), p. 982-989 |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | openAccess |
Appears in Collections: | CIIMAR - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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Albuquerque L_2018.pdf | 1.13 MB | Adobe PDF | View/Open |
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