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https://hdl.handle.net/10216/120537
Author(s): | Almeida C.M.R. Oliveira T. Reis I. Gomes C.R. Mucha A.P. |
Title: | Bacterial community dynamic associated with autochthonous bioaugmentation for enhanced Cu phytoremediation of salt-marsh sediments |
Publisher: | Elsevier |
Issue Date: | 2017 |
Abstract: | Autochthonous bioaugmentation for metal phytoremediation is still little explored, particularly its application to estuarine salt marshes, but results obtained so far are promising. Nevertheless, understanding the behaviour of the microbial communities in the process of bioaugmentation and their role in improving metal phytoremediation is very important to fully validate the application of this biological technology. This study aimed to characterize the bacterial community dynamic associated with the application of autochthonous bioaugmentation in an experimentation which showed that Phragmites australis rhizosphere microorganisms could increase this salt marsh plant potential to phytoremediate Cu contaminated sediments. Bacterial communities present in the autochthonous microbial consortium resistant to Cu added to the medium and in the sediment at the beginning and at the end of the experiment were characterized by ARISA. Complementarily, the consortium and the sediment used for its production were characterized by next generation sequencing using the pyrosequencing platform 454. The microbial consortium resistant to Cu obtained from non-vegetated sediment was dominated by the genus Lactococcus (46%), Raoultella (25%), Bacillus (12%) and Acinetobacter (11%), whereas the one obtained form rhizosediment was dominated by the genus Gluconacetobacter (77%), Bacillus (17%) and Dyella (3%). Results clearly showed that, after two months of experiment, Cu caused a shift in the bacterial community structure of sediments, an effect that was observed either with or without addition of the metal resistant microbial consortium. Therefore, bioaugmentation application improved the process of phytoremediation (metal translocation by the plant was increased) without inducing long term changes in the bacterial community structure of the sediments. So, phytoremediation combined with autochthonous bioaugmentation can be a suitable technology for the recovery of estuarine areas, contributing for an efficient risk management strategy of these coastal zones. © 2017 |
Subject: | Bacteriology Bioremediation Coastal zones Estuaries Metals Microorganisms Risk management Sediments Social sciences Soil pollution control Soils Wetlands Bacterial community Bacterial community structure Estuarine areas Microbial communities Microbial consortia Next-generation sequencing Rhizosphere microorganisms Risk management strategies Bacteria copper copper bacterium community dynamics community structure copper estuarine sediment microorganism phytoremediation saltmarsh sediment pollution Acinetobacter Article Bacillus bioaugmentation community dynamics controlled study Dyella Gluconacetobacter Lactococcus microbial community microbial consortium next generation sequencing nonhuman Phragmites australis phytoremediation pyrosequencing Raoultella salt marsh analysis bacterium bioremediation chemistry classification estuary growth, development and aging metabolism microbiology Poaceae sediment water pollutant wetland Acinetobacter Bacillus (bacterium) Bacteria (microorganisms) Dyella Gluconacetobacter Lactococcus Phragmites australis Raoultella Bacteria Biodegradation, Environmental Copper Estuaries Geologic Sediments Microbial Consortia Poaceae Water Microbiology Water Pollutants, Chemical Wetlands |
URI: | https://hdl.handle.net/10216/120537 |
Source: | Marine Environmental Research, vol. 132, p. 68-78 |
Related Information: | info:eu-repo/grantAgreement/FCT/5876/147268/PT |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | CIIMAR - Artigo em Revista Científica Internacional |
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