Item Details

title

IN VITRO MODEL OF GLIOBLASTOMA TO STUDY THE EFFECTS OF BRAIN MICROENVIRONMENT AND ITS MECHANICAL PROPERTIES ON TUMOR CELLS

author

Sivakumar, Hemamylammal

abstract

Glioblastoma (GBM) is one of most aggressive forms of brain cancer with a median survival rate of 14.6 months, and the two-year survival rate is 30%, which has not seen significant improvement in the last 100 years. This low survival rate could be in part attributed to the fact that there is a lack of model systems of this type of cancer which faithfully recapitulate the tumor architecture and microenvironment seen in vivo in humans, in which therapeutic studies would provide results that could be translated to the clinic efficiently. The two aims of this project are a) to create a bioengineered 3D in vitro model of GBM that addresses the gap mentioned above, and in turn could be used in the future for drug screening and personalized medicine to test drug responsiveness and monitor disease progression of the tumor, and b) to assess the role of the tumor microenvironment physical parameters on the tumor and its potential use as a biomarker. We conducted experiments to assess the sensitivity in different glioblastoma cell lines with different mutations to the elastic modulus of the microenvironment.

subject

elastic modulus

epidermal growth factor receptor

Glioblastoma

organoid

tumor heterogeneity

contributor

Skardal, Aleksander (committee chair)

Soker, Shay (committee member)

Strowd, Roy E (committee member)

date

2017-06-15T08:35:56Z (accessioned)

2017-12-14T09:30:10Z (available)

2017 (issued)

degree

Biomedical Science – MS (discipline)

embargo

2017-12-14 (terms)

identifier

http://hdl.handle.net/10339/82198 (uri)

language

en (iso)

publisher

Wake Forest University

type

Thesis