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Title: | ZRSR1 co-operates with ZRSR2 in regulating splicing of U12-type introns in murine hematopoietic cells | Authors: | Madan, Vikas Cao, Zeya Teoh, Weoi Woon Dakle, Pushkar Han, Lin Shyamsunder, Pavithra Jeitany, Maya Zhou, Siqin Li, Jia Hazimah Mohd Nordin Shi, JiZhong Yu, Shuizhou Yang, Henry Md Zakir Hossain Chng, Wee Joo Koeffler, H Phillip |
Keywords: | Science::Medicine | Issue Date: | 2022 | Source: | Madan, V., Cao, Z., Teoh, W. W., Dakle, P., Han, L., Shyamsunder, P., Jeitany, M., Zhou, S., Li, J., Hazimah Mohd Nordin, Shi, J., Yu, S., Yang, H., Md Zakir Hossain, Chng, W. J. & Koeffler, H. P. (2022). ZRSR1 co-operates with ZRSR2 in regulating splicing of U12-type introns in murine hematopoietic cells. Haematologica, 107(3), 680-689. https://dx.doi.org/10.3324/haematol.2020.260562 | Project: | NMRC/CG/012/2013 MOE2014-T3-1-006 |
Journal: | Haematologica | Abstract: | Recurrent loss-of-function mutations of spliceosome gene, ZRSR2, occur in myelodysplastic syndromes (MDS). Mutation/loss of ZRSR2 in human myeloid cells primarily causes impaired splicing of the U12-type introns. In order to further investigate the role of this splice factor in RNA splicing and hematopoietic development, we generated mice lacking ZRSR2. Unexpectedly, Zrsr2-deficient mice developed normal hematopoiesis with no abnormalities in myeloid differentiation evident in either young or ≥1-year old knockout mice. Repopulation ability of Zrsr2-deficient hematopoietic stem cells was also unaffected in both competitive and non-competitive reconstitution assays. Myeloid progenitors lacking ZRSR2 exhibited mis-splicing of U12-type introns, however, this phenotype was moderate compared to the ZRSR2-deficient human cells. Our investigations revealed that a closely related homolog, Zrsr1, expressed in the murine hematopoietic cells, but not in human cells contributes to splicing of U12-type introns. Depletion of Zrsr1 in Zrsr2 KO myeloid cells exacerbated retention of the U12-type introns, thus highlighting a collective role of ZRSR1 and ZRSR2 in murine U12-spliceosome. We also demonstrate that aberrant retention of U12-type introns of MAPK9 and MAPK14 leads to their reduced protein expression. Overall, our findings highlight that both ZRSR1 and ZRSR2 are functional components of the murine U12-spliceosome, and depletion of both proteins is required to accurately model ZRSR2-mutant MDS in mice. | URI: | https://hdl.handle.net/10356/162693 | ISSN: | 0390-6078 | DOI: | 10.3324/haematol.2020.260562 | Schools: | School of Biological Sciences | Organisations: | Cancer Science Institute of Singapore, NUS | Rights: | © 2022 Ferrata Storti Foundation. Material published in Haematologica is covered by copyright. All rights are reserved to the Ferrata Storti Foundation. Use of published material is allowed under the following terms and conditions: https://creativecommons.org/licenses/by-nc/4.0/legalcode. Copies of published material are allowed for personal or internal use. Sharing published material for non-commercial purposes is subject to the following conditions: https://creativecommons.org/licenses/by-nc/4.0/legalcode, sect. 3. Reproducing and sharing published material for commercial purposes is not allowed without permission in writing from the publisher. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Journal Articles |
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