Utilize este identificador para referenciar este registo: http://hdl.handle.net/10362/158546
Título: Safety of Gold Nanoparticles
Autor: Lopes, Joana
Ferreira-Gonçalves, Tânia
Ascensão, Lia
Viana, Ana S.
Carvalho, Lina
Catarino, José
Faísca, Pedro
Oliva, Abel
de Barros, Dragana P.C.
Rodrigues, Cecília M.P.
Gaspar, Maria Manuela
Reis, Catarina Pinto
Palavras-chave: cancer
ex vivo models
gold nanoparticles
in vitro models
in vivo models
nanomedicine
nanotoxicology
Pharmaceutical Science
SDG 3 - Good Health and Well-being
Data: Abr-2023
Resumo: In recent years, gold nanoparticles (AuNPs) have aroused the interest of many researchers due to their unique physicochemical and optical properties. AuNPs are being explored in a variety of biomedical fields, either in diagnostics or therapy, particularly for localized thermal ablation of cancer cells after light irradiation. Besides the promising therapeutic potential of AuNPs, their safety constitutes a highly important issue for any medicine or medical device. For this reason, in the present work, the production and characterization of physicochemical properties and morphology of AuNPs coated with two different materials (hyaluronic and oleic acids (HAOA) and bovine serum albumin (BSA)) were firstly performed. Based on the above importantly referred issue, the in vitro safety of developed AuNPs was evaluated in healthy keratinocytes, human melanoma, breast, pancreatic and glioblastoma cancer cells, as well as in a three-dimensional human skin model. Ex vivo and in vivo biosafety assays using, respectively, human red blood cells and Artemia salina were also carried out. HAOA-AuNPs were selected for in vivo acute toxicity and biodistribution studies in healthy Balb/c mice. Histopathological analysis showed no significant signs of toxicity for the tested formulations. Overall, several techniques were developed in order to characterize the AuNPs and evaluate their safety. All these results support their use for biomedical applications.
Descrição: Funding Information: The authors acknowledge Fundação para a Ciência e a Tecnologia (FCT) for financial support through Projects UIDB/00645/2020, UIDB/04138/2020, UIDP/04138/2020, PTDC/BTM-MAT/31794/2017, and PTDC/QUI-QIN/0586/2020, as well as PhD fellowships SFRH/BD/148044/2019 and SFRH/BD/147306/2019. The authors are also thankful for the financial support to CESAM by FCT/MCTES (UIDP/50017/2020+UIDB/50017/2020+LA/P/0094/2020+UIDB/00100/2020) through national funds. Publisher Copyright: © 2023 by the authors.
Peer review: yes
URI: http://hdl.handle.net/10362/158546
DOI: https://doi.org/10.3390/pharmaceutics15041120
ISSN: 1999-4923
Aparece nas colecções:Home collection (ITQB)

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