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Crystallization and preliminary X-ray crystallographic studies of response regulator for cyanobacterial phytochrome, Rcp1
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Cited 1 time in Scopus
- Authors
- Issue Date
- 2000-11
- Publisher
- Blackwell Publishing Inc.
- Citation
- Acta Crystallographica Section D: Biological Crystallography, Vol.56, pp.1446-1448
- Abstract
- The key response-regulator gene of light regulation, rcp1, from Synechocystis sp. has been overexpressed, purified and subsequently crystallized using ammonium sulfate as a precipitant in forms suitable for X-ray crystallographic studies. A native data set was collected to a resolution of 2.5 Angstrom at cryogenic temperature. The crystals belong to the hexagonal space group P6(3), with unit-cell parameters a = b = 89.04 (5), c = 60.29 (3) Angstrom. The Matthews parameter suggests that Rcp1 crystallizes with two molecules per asymmetric unit.
- ISSN
- 0907-4449
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