Publications
Detailed Information
In Vivo Imaging of Sentinel Nodes Using Fluorescent Silica Nanoparticles in Living Mice
Cited 22 time in
Web of Science
Cited 27 time in Scopus
- Authors
- Issue Date
- 2010-04
- Publisher
- SPRINGER
- Citation
- MOLECULAR IMAGING AND BIOLOGY; Vol.12 2; 155-162
- Keywords
- Silica nanoparticles ; Sentinel node ; Optical imaging
- Abstract
- We examine the feasibility of fluorescent imaging system for sentinel lymph node detection by using functionalized silica nanoparticles. We developed a functionalized RITC-SiO(2) nanoparticles containing fluorescent dye, C(28)H(31)N(2)O(3)Cl (rhodamine B isothiocyanate) inside, and subsequently synthesized (68)Ga-NOTA-RITC-SiO(2) nanoparticles. At 5 min after RITC-doped silica nanoparticles injection, fluorescent signals were shown in both right axillary lymph node (ALN) and injection site of living mice. Fluorescent signals were also observed at these locations in a biodistribution study. In addition, fluorescence was detected in frozen ALN sections microscopically. The percentages of doses injected per gram of tissue of axillary and brachial lymph nodes near footpad treated with (68)Ga-NOTA-RITC-SiO(2) nanoparticles were 308.3 +/- 3.4 and 41.5 +/- 6.1, respectively. Little (68)Ga radioactivity was found in other organs. Our data provide strong evidence that functionalized silica nanoparticles has a promising potential as organic lymphatic tracer in biomedical imaging such as pre- and intraoperative surgical guidance.
- ISSN
- 1536-1632
- Language
- English
- Files in This Item:
- There are no files associated with this item.
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.