급성 일산화탄소 중독이 백서소뇌 Purkinje 세포에 미치는 영향에 관한 세포병리학적 연구

Abstract

An experimental study was performed to observe the cytopathologic effects of acute carbon monoxide intoxication in lapse on the cerebellar Purkinje cells 。f rats by means of cytologic, enzyme cytochemical methods using light and electron microscope attem' pting to explore the pathogenesis related to ultrastr uctural features and enzyme cytochemistry. Experimental animals(125 Wister rats , 300-350gm.) were devided into control and exposure groups which were exposed by 0.5%CO for 45 min. And thereafter left to fresh air in 15 minutes through 30 days past to acute carbon monoxide intoxication. Cytopathologic investigation around the stainability, changes of Nissl substances and ultrastructural study on Purlinje cells of rat cerebellum were made. and enzyme activities of succinic dehydrogenase, NADH diaphorase, lactic dehydrogenase, acid phosphatase and thiamine pyrophosphatase in them were observed by appropriate enzyme cytochemical stainings. Results obtained were as follows: 1. The blood CO-Hb concentration was 89. 1±7. 6 % in acute carbon monoxide intoxication with 0.5% CO for 45 min. exposure and then rapidly degraded to 20. 2±9. 4% in 60 min. past the intoxication to leave in fresh air and reached to normal range in 4 hours past 2. Histologic sections of Nissl stainings revealed moderate decrease of stainability in chromatophilia of Nissl substances of cerebellar Purkinje cells and mild eloudy swelling of them with occasional central chromatolysis in rats of acute carbon monoxide intoxication. These findings were aggravated as far as 2 hours past the intoxication and thereafter arnended gradually in 6 hours through 24 hours pas t. 3. a) Mitochondrial SDH and NADH diaphorase activities of cerebellar Purkinje cells were moderately decreased in acute carbon monoxide intoxication, and their activities were gradually increased in 60 min. through 24 hours past the intoxication and thereafter reached to normal activities as control, to be more or less distinct changes in the midzonal area of their cytoplasm