Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy

Zhanghao, K; Chen, X; Liu, W; Li, M; Liu, Y; Wang, Y; Luo, S; Wang, X; Shan, C; Xie, H; Gao, J; Jin, D; Li, X; Zhang, Y; Dai, Q; Xi, P

Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy

Zhanghao, K

Chen, X Liu, W

Li, M Liu, Y Wang, Y Luo, S Wang, X Shan, C Xie, H Gao, J Jin, D

Li, X

Zhang, Y Dai, Q Xi, P

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Publication Type:: Journal Article
Citation:: Nature Communications, 2019, 10 (1)
Issue Date:: 2019-12-01

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Field	Value	Language
dc.contributor.author	Zhanghao, K https://orcid.org/0000-0002-8331-6240	en_US
dc.contributor.author	Chen, X	en_US
dc.contributor.author	Liu, W https://orcid.org/0000-0002-3003-1313	en_US
dc.contributor.author	Li, M	en_US
dc.contributor.author	Liu, Y	en_US
dc.contributor.author	Wang, Y	en_US
dc.contributor.author	Luo, S	en_US
dc.contributor.author	Wang, X	en_US
dc.contributor.author	Shan, C	en_US
dc.contributor.author	Xie, H	en_US
dc.contributor.author	Gao, J	en_US
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666	en_US
dc.contributor.author	Li, X https://orcid.org/0000-0001-8677-9833	en_US
dc.contributor.author	Zhang, Y	en_US
dc.contributor.author	Dai, Q	en_US
dc.contributor.author	Xi, P https://orcid.org/0000-0001-6626-4840	en_US
dc.date.available	2019-09-20	en_US
dc.date.issued	2019-12-01	en_US
dc.identifier.citation	Nature Communications, 2019, 10 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/136577
dc.description.abstract	© 2019, The Author(s). Fluorescence polarization microscopy images both the intensity and orientation of fluorescent dipoles and plays a vital role in studying molecular structures and dynamics of bio-complexes. However, current techniques remain difficult to resolve the dipole assemblies on subcellular structures and their dynamics in living cells at super-resolution level. Here we report polarized structured illumination microscopy (pSIM), which achieves super-resolution imaging of dipoles by interpreting the dipoles in spatio-angular hyperspace. We demonstrate the application of pSIM on a series of biological filamentous systems, such as cytoskeleton networks and λ-DNA, and report the dynamics of short actin sliding across a myosin-coated surface. Further, pSIM reveals the side-by-side organization of the actin ring structures in the membrane-associated periodic skeleton of hippocampal neurons and images the dipole dynamics of green fluorescent protein-labeled microtubules in live U2OS cells. pSIM applies directly to a large variety of commercial and home-built SIM systems with various imaging modality.	en_US
dc.relation.ispartof	Nature Communications	en_US
dc.relation.isbasedon	10.1038/s41467-019-12681-w	en_US
dc.subject.mesh	Kidney	en_US
dc.subject.mesh	Hippocampus	en_US
dc.subject.mesh	Neurons	en_US
dc.subject.mesh	Cell Line, Tumor	en_US
dc.subject.mesh	Cytoskeleton	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Bacteriophage lambda	en_US
dc.subject.mesh	Actins	en_US
dc.subject.mesh	Myosins	en_US
dc.subject.mesh	DNA	en_US
dc.subject.mesh	Microscopy, Fluorescence	en_US
dc.subject.mesh	Microscopy, Polarization	en_US
dc.title	Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	10	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Computer Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - AAI - Advanced Analytics Institute Research Centre
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	10	en_US

Abstract:

© 2019, The Author(s). Fluorescence polarization microscopy images both the intensity and orientation of fluorescent dipoles and plays a vital role in studying molecular structures and dynamics of bio-complexes. However, current techniques remain difficult to resolve the dipole assemblies on subcellular structures and their dynamics in living cells at super-resolution level. Here we report polarized structured illumination microscopy (pSIM), which achieves super-resolution imaging of dipoles by interpreting the dipoles in spatio-angular hyperspace. We demonstrate the application of pSIM on a series of biological filamentous systems, such as cytoskeleton networks and λ-DNA, and report the dynamics of short actin sliding across a myosin-coated surface. Further, pSIM reveals the side-by-side organization of the actin ring structures in the membrane-associated periodic skeleton of hippocampal neurons and images the dipole dynamics of green fluorescent protein-labeled microtubules in live U2OS cells. pSIM applies directly to a large variety of commercial and home-built SIM systems with various imaging modality.

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http://hdl.handle.net/10453/136577