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A hybrid FIA/HPLC system incorporating monolithic column chromatography
journal contribution
posted on 2007-09-02, 00:00 authored by Jacqui AdcockJacqui Adcock, Paul FrancisPaul Francis, Kent Mitchell Agg, G Marshall, Neil BarnettNeil BarnettWe have combined the generation of solvent gradients using milliGAT pumps, chromatographic separations with monolithic columns and chemiluminescence detection in an instrument manifold that approaches the automation and separation efficiency of HPLC, whilst maintaining the positive attributes of flow injection analysis (FIA), such as manifold versatility, speed of analysis and portability. As preliminary demonstrations of this hybrid FIA/HPLC system, we have determined six opiate alkaloids (morphine, pseudomorphine, codeine, oripavine, ethylmorphine and thebaine) and four biogenic amines (vanilmandelic acid, serotonin, 5-hydroxyindole-3-acetic acid and homovanillic acid) in human urine, using tris(2,2′-bipyridyl)ruthenium(III) and acidic potassium permanganate chemiluminescence detection.
History
Journal
Analytica chimica actaVolume
600Issue
1-2Pagination
136 - 141Publisher
Elsevier BVLocation
Amsterdam, NetherlandsPublisher DOI
ISSN
0003-2670eISSN
1873-4324Language
engNotes
Available online 25 March 2007.Publication classification
C1 Refereed article in a scholarly journal; C Journal articleCopyright notice
2007, Elsevier B.V.Usage metrics
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No categories selectedKeywords
flow injection analysisgradient elutionmonolithic column chromatographychemiluminescence detectionneurotransmitter metabolitesScience & TechnologyPhysical SciencesChemistry, AnalyticalChemistryFLOW-INJECTION ANALYSISPERFORMANCE LIQUID-CHROMATOGRAPHYSEQUENTIAL INJECTIONPHARMACEUTICAL-PREPARATIONSAMBROXOL HYDROCHLORIDEAMINO-ACIDSSEPARATIONSILICAMETHYLPARABEN
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